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High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiĀ­amine pyrophosphate

DOI: 10.1107/S2053230X1501657X DOI Help
PMID: 26457526 PMID Help

Authors: Petra Lukacik (Diamond Light Source) , Carina Lobley (Diamond Light Source) , Mario Bumann (MRC France) , Victoria Arena De Souza (Oxford Protein Production Facility UK) , Raymond J. Owens (Oxford Protein Production Facility UK) , Paul W. O'toole (MRC France) , Martin A. Walsh (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 71 , PAGES 1327 - 1334

State: Published (Approved)
Published: October 2015

Open Access Open Access

Abstract: Probiotic bacterial strains have been shown to enhance the health of the host through a range of mechanisms including colonization, resistance against pathogens, secretion of antimicrobial compounds and modulation of the activity of the innate immune system. Lactobacillus salivarius UCC118 is a well characterized probiotic strain which survives intestinal transit and has many desirable host-interaction properties. Probiotic bacteria display a wide range of catabolic activities, which determine their competitiveness in vivo. Some lactobacilli are heterofermentative and can metabolize pentoses, using a pathway in which transketolase and transaldolase are key enzymes. L. salivarius UCC118 is capable of pentose utilization because it encodes the key enzymes on a megaplasmid. The crystal structures of the megaplasmid-encoded transketolase with and without the enzyme cofactor thiamine pyrophosphate have been determined. Comparisons with other known transketolase structures reveal a high degree of structural conservation in both the catalytic site and the overall conformation. This work extends structural knowledge of the transketolases to the industrially and commercially important Lactobacillus genus.

Keywords: transketolase; Lactobacillus salivarius

Subject Areas: Biology and Bio-materials

Beamlines: I04-1-Macromolecular Crystallography (fixed wavelength)