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Instruments / Technical Area	Publication Details	Published	Actions
Talos-Talos Arctica at Diamond	Machining protein microcrystals for structure determination by electron diffraction Helen M. E. Duyvesteyn , Abhay Kotecha , Helen M. Ginn , Corey W. Hecksel , Emma V. Beale , Felix De Haas , Gwyndaf Evans , Peijun Zhang , Wah Chiu , David I. Stuart Proceedings Of The National Academy Of Sciences 74 DOI: 10.1073/pnas.1809978115 Open Access Show Abstract ▼ Abstract: We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.	Aug 2018

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