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Instruments / Technical Area	Publication Details	Published	Actions
Krios I-Titan Krios I at Diamond	A workflow for protein structure determination from thin crystal lamella by micro-electron diffraction Emma V. Beale , David G. Waterman , Corey Hecksel , Jason Van Rooyen , James B. Gilchrist , James M. Parkhurst , Felix De Haas , Bart Buijsse , Gwyndaf Evans , Peijun Zhang Frontiers In Molecular Biosciences 7 DOI: 10.3389/fmolb.2020.00179 Diamond Proposal Number(s): [23169] Open Access Show Abstract ▼ Abstract: MicroED has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nanometers in thickness. Furthermore, traditional microED data processing uses established X-ray crystallography software that is not optimized for handling compound effects that are unique to electron diffraction data. Here, we present an integrated workflow for microED, from sample preparation by cryo-focused ion beam milling, through data collection with a standard Ceta-D detector, to data processing using the DIALS software suite, thus enabling routine atomic structure determination of protein crystals of any size and shape using microED. We demonstrate the effectiveness of the workflow by determining the structure of proteinase K to 2.0 Å resolution and show the advantage of using protein crystal lamellae over nanocrystals.	Aug 2020

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Krios I-Titan Krios I at Diamond

A workflow for protein structure determination from thin crystal lamella by micro-electron diffraction

Emma V. Beale , David G. Waterman , Corey Hecksel , Jason Van Rooyen , James B. Gilchrist , James M. Parkhurst , Felix De Haas , Bart Buijsse , Gwyndaf Evans , Peijun Zhang

Frontiers In Molecular Biosciences 7

DOI: 10.3389/fmolb.2020.00179

Diamond Proposal Number(s): [23169]