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Instruments / Technical Area	Publication Details	Published	Actions
I04-Macromolecular Crystallography	Hybrid assemblies of a symmetric designer protein and polyoxometalates with matching symmetry Laurens Vandebroek , Hiroki Noguchi , Kenichi Kamata , Jeremy R. H. Tame , Luc Van Meervelt , Tatjana N. Parac-Vogt , Arnout R. D. Voet Chemical Communications 56, 11601 - 11604 DOI: 10.1039/D0CC05071G Show Abstract ▼ Abstract: Novel bioinorganic hybrid materials based on proteins and inorganic clusters have enormous potential for the development of hybrid catalysts that synergistically combine properties of both materials. Here we report the creation of hybrid assemblies between a computationally designed symmetrical protein Pizza6-S and different polyoxometalates with matching symmetry: the tellurotungstic Anderson-Evans (Na6[TeW6O24]·22H2O) (TEW); Keggin (H4[SiW12O40]·6H2O) (STA); and 1[thin space (1/6-em)]:[thin space (1/6-em)]2 CeIII-substituted Keggin (K11[CeIII[PW11O39]2]·20H2O) (Ce-K). This results in the formation of complexes with clearly defined stoichiometries in solution. Crystal structures validate the complexes as building blocks for the formation of larger assemblies.	Oct 2020
I04-Macromolecular Crystallography	Structural plasticity of a designer protein sheds light on β ‐propeller protein evolution Bram Mylemans , Ina Laier , Kenichi Kamata , Satoko Akashi , Hiroki Noguchi , Jeremy R. H. Tame , Arnout R. D. Voet The Febs Journal DOI: 10.1111/febs.15347 Diamond Proposal Number(s): [19190] Show Abstract ▼ Abstract: β‐propeller proteins are common in nature, where they are observed to adopt 4‐ to 10‐fold internal rotational pseudosymmetry. This size diversity can be explained by the evolutionary process of gene duplication and fusion. In this study we investigated a distorted β‐propeller protein, an apparent intermediate between two symmetries. From this template, we created a perfectly symmetric 9‐bladed β‐propeller named Cake, using computational design and ancestral sequence reconstruction. The designed repeat sequence was found to be capable of generating both 8‐fold and 9‐fold propellers which are highly stable. Cake variants with 2 to 10 identical copies of the repeat sequence were characterized by X‐ray crystallography and in solution. They were found to be highly stable, and to self‐assemble into 8‐ or 9‐ fold symmetrical propellers. These findings show that the β‐propeller fold allows sufficient structural plasticity to permit a given blade to assemble different forms, a transition from even to odd changes in blade number, and provide a potential explanation for the wide diversity of repeat numbers observed in natural propeller proteins.	Apr 2020

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