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Instruments / Technical Area	Publication Details	Published	Actions
Krios I-Titan Krios I at Diamond Krios II-Titan Krios II at Diamond Krios III-Titan Krios III at Diamond Krios IV-Titan Krios IV at Diamond Talos-Talos Arctica at Diamond	Single particle cryo-electron microscopy: From sample to structure Joshua B. R. White , Daniel P. Maskell , Andrew Howe , Martin Harrow , Daniel K. Clare , C. Alistair Siebert , Emma L. Hesketh , Rebecca F. Thompson Journal Of Visualized Experiments DOI: 10.3791/62415 Diamond Proposal Number(s): [23047] Open Access Show Abstract ▼ Abstract: Cryo-electron microscopy (cryoEM) is a powerful technique for structure determination of macromolecular complexes, via single particle analysis (SPA). The overall process involves i) vitrifying the specimen in a thin film supported on a cryoEM grid; ii) screening the specimen to assess particle distribution and ice quality; iii) if the grid is suitable, collecting a single particle dataset for analysis; and iv) image processing to yield an EM density map. In this protocol, an overview for each of these steps is provided, with a focus on the variables which a user can modify during the workflow and the troubleshooting of common issues. With remote microscope operation becoming standard in many facilities, variations on imaging protocols to assist users in efficient operation and imaging when physical access to the microscope is limited will be described.	May 2021

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