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Instruments / Technical Area	Publication Details	Published	Actions
I14-Hard X-ray Nanoprobe	The Delta Robot—A long travel nano-positioning stage for scanning x-ray microscopy Jon Kelly , Andrew Male , Nicholas Rubies , David Mahoney , Jessica Walker , Miguel A. Gomez-Gonzalez , Guy Wilkin , Julia E. Parker , Paul D. Quinn Review Of Scientific Instruments 93 DOI: 10.1063/5.0084806 Open Access Show Abstract ▼ Abstract: A new stage design concept, the Delta Robot, is presented, which is a parallel kinematic design for scanning x-ray microscopy applications. The stage employs three orthogonal voice coils, which actuate parallelogram flexures. The design has a 3 mm travel range and achieves rms position jitter, integrated from 1 Hz to 1 kHz, of 2.8 and 1.3 nm perpendicular to the beam and 5.6 nm along the beam direction with loads up to 350 g. The Delta Robot design process used a mechatronics approach of iterative modeling and simulation to develop the system and validate performance. The design considerations, design process, stability, and operational performance on the hard x-ray nanoprobe at Diamond Light Source are presented.	Apr 2022
Krios II-Titan Krios II at Diamond	A CDK-regulated chromatin segregase promoting chromosome replication Erika Chacin , Priyanka Bansal , Karl-Uwe Reusswig , Luis M. Diaz-Santin , Pedro Ortega , Petra Vizjak , Belen Gomez-Gonzalez , Felix Müller-Planitz , Andrés Aguilera , Boris Pfander , Alan C. M. Cheung , Christoph F. Kurat Nature Communications 12 DOI: 10.1038/s41467-021-25424-7 Diamond Proposal Number(s): [20287] Open Access Show Abstract ▼ Abstract: The replication of chromosomes during S phase is critical for cellular and organismal function. Replicative stress can result in genome instability, which is a major driver of cancer. Yet how chromatin is made accessible during eukaryotic DNA synthesis is poorly understood. Here, we report the characterization of a chromatin remodeling enzyme—Yta7—entirely distinct from classical SNF2-ATPase family remodelers. Yta7 is a AAA+ -ATPase that assembles into ~1 MDa hexameric complexes capable of segregating histones from DNA. The Yta7 chromatin segregase promotes chromosome replication both in vivo and in vitro. Biochemical reconstitution experiments using purified proteins revealed that the enzymatic activity of Yta7 is regulated by S phase-forms of Cyclin-Dependent Kinase (S-CDK). S-CDK phosphorylation stimulates ATP hydrolysis by Yta7, promoting nucleosome disassembly and chromatin replication. Our results present a mechanism for how cells orchestrate chromatin dynamics in co-ordination with the cell cycle machinery to promote genome duplication during S phase.	Sep 2021

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