Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS	The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Juan L Benavente , Dritan Siliqi , Lourdes Infantes , Laura Lagartera , Alberto Mills , Federico Gago , Noemí Ruiz-López , Miguel A Botella , Maria J. Sanchez-Barrena , Armando Albert Life Science Alliance 4 DOI: 10.26508/lsa.202101152 Diamond Proposal Number(s): [21741] Open Access Show Abstract ▼ Abstract: Non-vesicular lipid transfer at ER and plasma membrane (PM) contact sites (CS) is crucial for the maintenance of membrane lipid homeostasis. Extended synaptotagmins (E-Syts) play a central role in this process as they act as molecular tethers of ER and PM and as lipid transfer proteins between these organelles. E-Syts are proteins constitutively anchored to the ER through an N-terminal hydrophobic segment and bind the PM via a variable number of C-terminal C2 domains. Synaptotagmins (SYTs) are the plant orthologous of E-Syts and regulate the ER–PM communica- tion in response to abiotic stress. Combining different structural and biochemical techniques, we demonstrate that the binding of SYT1 to lipids occurs through a Ca2+-dependent lipid-binding site and by a site for phosphorylated forms of phosphatidylinositol, thus integrating two different molecular signals in response to stress. In addition, we show that SYT1 displays three highly flexible hinge points that provide conformational freedom to facilitate lipid extraction, protein loading, and subsequent transfer between PM and ER.	Aug 2021

Instruments / Technical Area

Publication Details

Published

Actions

B21-High Throughput SAXS

The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites

Juan L Benavente , Dritan Siliqi , Lourdes Infantes , Laura Lagartera , Alberto Mills , Federico Gago , Noemí Ruiz-López , Miguel A Botella , Maria J. Sanchez-Barrena , Armando Albert

Life Science Alliance 4

DOI: 10.26508/lsa.202101152

Diamond Proposal Number(s): [21741]