|
Austin
Echelmeier
,
Jorvani
Cruz Villarreal
,
Marc
Messerschmidt
,
Daihyun
Kim
,
Jesse D.
Coe
,
Darren
Thifault
,
Sabine
Botha
,
Ana
Egatz-gomez
,
Sahir
Gandhi
,
Gerrit
Brehm
,
Chelsie E.
Conrad
,
Debra T.
Hansen
,
Caleb
Madsen
,
Saša
Bajt
,
J. Domingo
Meza-aguilar
,
Dominik
Oberthuer
,
Max O.
Wiedorn
,
Holger
Fleckenstein
,
Derek
Mendez
,
Juraj
Knoška
,
Jose M.
Martin-garcia
,
Hao
Hu
,
Stella
Lisova
,
Aschkai
Allahgoli
,
Yaroslav
Gevorkov
,
Kartik
Ayyer
,
Steve
Aplin
,
Helen M.
Ginn
,
Heinz
Graafsma
,
Andrew J.
Morgan
,
Dominic
Greiffenberg
,
Alexander
Klujev
,
Torsten
Laurus
,
Jennifer
Poehlsen
,
Ulrich
Trunk
,
Davide
Mezza
,
Bernd
Schmitt
,
Manuela
Kuhn
,
Raimund
Fromme
,
Jolanta
Sztuk-dambietz
,
Natascha
Raab
,
Steffen
Hauf
,
Alessandro
Silenzi
,
Thomas
Michelat
,
Chen
Xu
,
Cyril
Danilevski
,
Andrea
Parenti
,
Leonce
Mekinda
,
Britta
Weinhausen
,
Grant
Mills
,
Patrik
Vagovic
,
Yoonhee
Kim
,
Henry
Kirkwood
,
Richard
Bean
,
Johan
Bielecki
,
Stephan
Stern
,
Klaus
Giewekemeyer
,
Adam
Round
,
Joachim
Schulz
,
Katerina
Dörner
,
Thomas D.
Grant
,
Valerio
Mariani
,
Anton
Barty
,
Adrian P.
Mancuso
,
Uwe
Weierstall
,
John C. H.
Spence
,
Henry N.
Chapman
,
Nadia
Zatsepin
,
Petra
Fromme
,
Richard A.
Kirian
,
Alexandra
Ros
Open Access
Abstract: Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.
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Sep 2020
|
|
I03-Macromolecular Crystallography
|
Max O.
Wiedorn
,
Dominik
Oberthuer
,
Richard
Bean
,
Robin
Schubert
,
Nadine
Werner
,
Brian
Abbey
,
Martin
Aepfelbacher
,
Luigi
Adriano
,
Aschkan
Allahgholi
,
Nasser
Al-qudami
,
Jakob
Andreasson
,
Steve
Aplin
,
Salah
Awel
,
Kartik
Ayyer
,
Saša
Bajt
,
Imrich
Barák
,
Sadia
Bari
,
Johan
Bielecki
,
Sabine
Botha
,
Djelloul
Boukhelef
,
Wolfgang
Brehm
,
Sandor
Brockhauser
,
Igor
Cheviakov
,
Matthew A.
Coleman
,
Francisco
Cruz-mazo
,
Cyril
Danilevski
,
Connie
Darmanin
,
R. Bruce
Doak
,
Martin
Domaracky
,
Katerina
Dörner
,
Yang
Du
,
Hans
Fangohr
,
Holger
Fleckenstein
,
Matthias
Frank
,
Petra
Fromme
,
Alfonso M.
Gañán-calvo
,
Yaroslav
Gevorkov
,
Klaus
Giewekemeyer
,
Helen Mary
Ginn
,
Heinz
Graafsma
,
Rita
Graceffa
,
Dominic
Greiffenberg
,
Lars
Gumprecht
,
Peter
Göttlicher
,
Janos
Hajdu
,
Steffen
Hauf
,
Michael
Heymann
,
Susannah
Holmes
,
Daniel A.
Horke
,
Mark S.
Hunter
,
Siegfried
Imlau
,
Alexander
Kaukher
,
Yoonhee
Kim
,
Alexander
Klyuev
,
Juraj
Knoška
,
Bostjan
Kobe
,
Manuela
Kuhn
,
Christopher
Kupitz
,
Jochen
Küpper
,
Janine Mia
Lahey-rudolph
,
Torsten
Laurus
,
Karoline
Le Cong
,
Romain
Letrun
,
P. Lourdu
Xavier
,
Luis
Maia
,
Filipe R. N. C.
Maia
,
Valerio
Mariani
,
Marc
Messerschmidt
,
Markus
Metz
,
Davide
Mezza
,
Thomas
Michelat
,
Grant
Mills
,
Diana C. F.
Monteiro
,
Andrew
Morgan
,
Kerstin
Mühlig
,
Anna
Munke
,
Astrid
Münnich
,
Julia
Nette
,
Keith A.
Nugent
,
Theresa
Nuguid
,
Allen M.
Orville
,
Suraj
Pandey
,
Gisel
Pena
,
Pablo
Villanueva-perez
,
Jennifer
Poehlsen
,
Gianpietro
Previtali
,
Lars
Redecke
,
Winnie Maria
Riekehr
,
Holger
Rohde
,
Adam
Round
,
Tatiana
Safenreiter
,
Iosifina
Sarrou
,
Tokushi
Sato
,
Marius
Schmidt
,
Bernd
Schmitt
,
Robert
Schönherr
,
Joachim
Schulz
,
Jonas A.
Sellberg
,
M. Marvin
Seibert
,
Carolin
Seuring
,
Megan L.
Shelby
,
Robert L.
Shoeman
,
Marcin
Sikorski
,
Alessandro
Silenzi
,
Claudiu A.
Stan
,
Xintian
Shi
,
Stephan
Stern
,
Jola
Sztuk-dambietz
,
Janusz
Szuba
,
Aleksandra
Tolstikova
,
Martin
Trebbin
,
Ulrich
Trunk
,
Patrik
Vagovic
,
Thomas
Ve
,
Britta
Weinhausen
,
Thomas A.
White
,
Krzysztof
Wrona
,
Chen
Xu
,
Oleksandr
Yefanov
,
Nadia
Zatsepin
,
Jiaguo
Zhang
,
Markus
Perbandt
,
Adrian P.
Mancuso
,
Christian
Betzel
,
Henry
Chapman
,
Anton
Barty
Open Access
Abstract: The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
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Oct 2018
|
|
I13-1-Coherence
Metrology
Optics
|
J.
Krempasky
,
U.
Flechsig
,
P.
Vagovic
,
C.
David
,
F.
Koch
,
A.
Jaggi
,
C.
Svetina
,
Shashidhara
Marathe
,
D.
Batey
,
S.
Cipiccia
,
C.
Rau
,
F.
Seiboth
,
M.
Seaberg
,
U. H.
Wagner
,
L.
Patthey
,
L.
Mikes
Diamond Proposal Number(s):
[19521]
Abstract: Preserving the coherence and wavefront of a diffraction limited x-ray beam from the source to the experiment poses stringent quality requirements on the production processes for X-ray optics. In the near future this will require on-line and in-situ at-wavelength metrology for both, free electron lasers and diffraction limited storage rings. A compact and easy to move X-ray grating interferometry (XGI) setup has been implemented by the Beamline Optics Group at PSI in order to characterize x-ray optical components by determining the aberrations from reconstructing the x-ray wavefront. The XGI setup was configured for measurements in the moire mode and tested with focusing optic at Swiss Light Source, Diamond Light Source and LCLS. In this paper measurements on a bendable toroidal mirror, a zone plate, a single and a stack of beryllium compound refractive lenses (CRL) are presented. From these measurements the focal position and quality of the beam spot in terms of wavefront distortions are determined by analysing the phase-signal obtained from the XGI measurement. In addition, using a bendable toroidal mirror, we directly compare radius of curvature measurements obtained from XGI data with data from a long-trace profilometer, and compare the CRL wavefront distortions with data obtained by ptychography.
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Sep 2018
|
|
Detectors
|
Abstract: We report on indirect X-ray detector systems for various full-field, ultra high-speed X-ray imaging methodologies, such as X-ray phase-contrast radiography, diffraction topography, grating interferometry and speckle-based imaging performed at the hard X-ray imaging beamline ID19 of the European Synchrotron—ESRF. Our work highlights the versatility of indirect X-ray detectors to multiple goals such as single synchrotron pulse isolation, multiple-frame recording up to millions frames per second, high efficiency, and high spatial resolution. Besides the technical advancements, potential applications are briefly introduced and discussed.
|
Apr 2018
|
|
B16-Test Beamline
|
B. K.
Tanner
,
J.
Wittge
,
P.
Vagovic
,
T.
Baumbach
,
D.
Allen
,
P. J.
Mcnally
,
R.
Bytheway
,
D.
Jacques
,
M. C.
Fossati
,
D. K.
Bowen
,
J.
Garagorri
,
M. R.
Elizalde
,
A. N.
Danilewsky
Abstract: The apparatus for X-ray diffraction imaging (XRDI) of 450-mm wafers, is now placed at the ANKA synchrotron radiation source in Karlsruhe, is described in the context of the drive to inspect wafers for plastic deformation or mechanical damage. It is shown that full wafer maps at high resolution can be expected to take a few hours to record. However, we show from experiments on 200-, 300-, and 450-mm wafers that a perimeter-scan on a 450-mm wafer, to pick up edge damage and edge-originated slip sources, can be achieved in just over 10 min. Experiments at the Diamond Light Source, on wafers still in their cassettes, suggest that clean-room conditions may not be necessary for such characterization. We conclude that scaling up of the 300-mm format Jordan Valley tools, together with the existing facility at ANKA, provides satisfactory capability for future XRDI analysis of 450-mm wafers.
|
Jun 2013
|
|
B16-Test Beamline
|
Patrik
Vagovic
,
Dusan
Korytar
,
Angelica
Cecilia
,
Elias
Hamann
,
Libor
Sveda
,
Daniele
Pelliccia
,
Jurgen
Hartwig
,
Peter
Oberta
,
Igor
Dolbnya
,
Kawal
Sawhney
,
Uwe
Fleschig
,
Michael
Fiederle
,
Tilo
Baumbach
Diamond Proposal Number(s):
[7662]
Abstract: The performance of a recently developed full-field X-ray micro-imaging system based on an in-line Bragg magnifier is reported. The system is composed of quasi-channel-cut crystals in combination with a Medipix single-photon-counting detector. A theoretical and experimental study of the imaging performance of the crystals-detector combination and a comparison with a standard indirect detector typically used in high-resolution X-ray imaging schemes are reported. The spatial resolution attained by our system is about 0.75 µm, limited only by the current magnification. Compared with an indirect detector system, this system features a better efficiency, signal-to-noise ratio and spatial resolution. The optimal working resolution range of this system is between 0.4 µm and 1 µm, filling the gap between transmission X-ray microscopes and indirect detectors. Applications for coherent full-field imaging of weakly absorbing samples are shown and discussed.
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Nov 2012
|
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