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Instruments / Technical Area	Publication Details	Published	Actions
Krios II-Titan Krios II at Diamond	Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics James Wagstaff , Vicente J. Planelles-Herrero , Grigory Sharov , Aisha Alnami , Frank Kozielski , Emmanuel Derivery , Jan Lowe Science Advances 9 DOI: 10.1126/sciadv.adf3021 Diamond Proposal Number(s): [23268] Open Access Show Abstract ▼ Abstract: Protein filaments are used in myriads of ways to organize other molecules within cells. Some filament-forming proteins couple the hydrolysis of nucleotides to their polymerization cycle, thus powering the movement of other molecules. These filaments are termed cytomotive. Only members of the actin and tubulin protein superfamilies are known to form cytomotive filaments. We examined the basis of cytomotivity via structural studies of the polymerization cycles of actin and tubulin homologs from across the tree of life. We analyzed published data and performed structural experiments designed to disentangle functional components of these complex filament systems. Our analysis demonstrates the existence of shared subunit polymerization switches among both cytomotive actins and tubulins, i.e., the conformation of subunits switches upon assembly into filaments. These cytomotive switches can explain filament robustness, by enabling the coupling of kinetic and structural polarities required for cytomotive behaviors and by ensuring that single cytomotive filaments do not fall apart.	Mar 2023
Krios I-Titan Krios I at Diamond	Using Scipion for stream image processing at Cryo-EM facilities J. Gómez-Blanco , J. M. De La Rosa-Trevín , R. Marabini , L. Del Cano , A. Jiménez , M. Martínez , R. Melero , T. Majtner , D. Maluenda , J. Mota , Y. Rancel , E. Ramírez-Aportela , J. I. Vilas , M. Carroni , S. Fleischmann , E. Lindahl , A. W. Ashton , M. Basham , D. K. Clare , K. Savage , C. A. Siebert , G. G. Sharov , C. O. S. Sorzano , P. Conesa , J. M. Carazo Journal Of Structural Biology DOI: 10.1016/j.jsb.2018.10.001 Open Access Show Abstract ▼ Abstract: Three dimensional electron microscopy is becoming a very data-intensive field in which vast amounts of experimental images are acquired at high speed. To manage such large-scale projects, we had previously developed a modular workflow system called Scipion (de la Rosa-Trevín et al., 2016). We present here a major extension of Scipion that allows processing of EM images while the data is being acquired. This approach helps to detect problems at early stages, saves computing time and provides users with a detailed evaluation of the data quality before the acquisition is finished. At present, Scipion has been deployed and is in production mode in seven Cryo-EM facilities throughout the world.	Oct 2018

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