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Instruments / Technical Area	Publication Details	Published	Actions
I13-2-Diamond Manchester Imaging	Spatiotemporal and microstructural characterization of heterotopic ossification in healing rat Achilles tendons Maria Pierantoni , Malin Hammerman , Isabella Silva Barreto , Daniel Larsson , Thomas Notermans , Andrew Bodey , Pernilla Eliasson , Hanna Isaksson The Faseb Journal 37 DOI: 10.1096/fj.202201018RRR Diamond Proposal Number(s): [16557] Open Access Show Abstract ▼ Abstract: Achilles tendon rupture is a common debilitating medical condition. The healing process is slow and can be affected by heterotopic ossification (HO), which occurs when pathologic bone-like tissue is deposited instead of the soft collagenous tendon tissue. Little is known about the temporal and spatial progression of HO during Achilles tendon healing. In this study we characterize HO deposition, microstructure, and location at different stages of healing in a rat model. We use phase contrast-enhanced synchrotron microtomography, a state-of-the-art technique that allows 3D imaging at high-resolution of soft biological tissues without invasive or time-consuming sample preparation. The results increase our understanding of HO deposition, from the early inflammatory phase of tendon healing, by showing that the deposition is initiated as early as one week after injury in the distal stump and mostly growing on preinjury HO deposits. Later, more deposits form first in the stumps and then all over the tendon callus, merging into large, calcified structures, which occupy up to 10% of the tendon volume. The HOs were characterized by a looser connective trabecular-like structure and a proteoglycan-rich matrix containing chondrocyte-like cells with lacunae. The study shows the potential of 3D imaging at high-resolution by phase-contrast tomography to better understand ossification in healing tendons.	Jun 2023

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I13-2-Diamond Manchester Imaging

Spatiotemporal and microstructural characterization of heterotopic ossification in healing rat Achilles tendons

Maria Pierantoni , Malin Hammerman , Isabella Silva Barreto , Daniel Larsson , Thomas Notermans , Andrew Bodey , Pernilla Eliasson , Hanna Isaksson

The Faseb Journal 37

DOI: 10.1096/fj.202201018RRR

Diamond Proposal Number(s): [16557]