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Instruments / Technical Area	Publication Details	Published	Actions
I14-Hard X-ray Nanoprobe	Imaging biomineralizing bacteria in the native-state with X-ray fluorescence microscopy Daniel M. Chevrier , Elisa Cerda-Donate , Lucia Gandarias , Miguel A. Gomez Gonzalez , Sufal Swaraj , Paul E. D. Soto Rodriguez , Antoine Fraisse , Tom Robinson , Damien Faivre Chemical Science DOI: 10.1039/D4SC08375J Diamond Proposal Number(s): [28688] Open Access Show Abstract ▼ Abstract: Understanding the interactions between metal-based nanoparticles and biological systems in complex environments (e.g., the human body, soils, and marine settings) remains challenging, especially at the single-cell and nanoscale levels. Capturing the dynamics of these interactions, such as metal distribution, nanoparticle growth, or degradation, in their native state (in vivo) is particularly difficult. Here, we demonstrate the direct measurement of iron content in hydrated, magnetite-biomineralizing magnetotactic bacteria using synchrotron-based nanobeam–scanning X-ray fluorescence microscopy combined with a liquid cell environment. In addition to X-ray fluorescence imaging, we collected iron chemical speciation information from individual bacteria in liquid using X-ray absorption spectroscopy. To follow biomineralization in situ, we developed a microfluidic device to track magnetite nanoparticle formation over several hours under the X-ray beam. This approach highlights the potential of X-ray fluorescence microscopy in liquid cell setups to provide elemental and chemical insights into biological processes at the single-cell level. Combining X-ray nanobeam techniques with liquid cell devices will enable more “on-chip” experiments on metals in biological contexts to be conducted at the synchrotron.	Mar 2025
I14-Hard X-ray Nanoprobe	Incorporation of Tb and Gd improves the diagnostic functionality of magnetotactic bacteria Lucia Gandarias , Elizabeth M. Jefremovas , David Gandia , Lourdes Marcano , Virginia Martínez-Martínez , Pedro Ramos-Cabrer , Daniel M. Chevrier , Sergio Valencia , Luis Fernández Barquín , M. Luisa Fdez-Gubieda , Javier Alonso , Ana García-Prieto , Alicia Muela Materials Today Bio 2 DOI: 10.1016/j.mtbio.2023.100680 Diamond Proposal Number(s): [28868] Open Access Show Abstract ▼ Abstract: Magnetotactic bacteria are envisaged as potential theranostic agents. Their internal magnetic compass, chemical specificity and natural motility enable these microorganisms to behave as nanorobots, as they can be tracked and guided towards specific regions and activated to generate a therapeutic response. Here we provide additional diagnostic functionalities to magnetotactic bacteria Magnetospirillum gryphiswaldense MSR-1 while retaining their intrinsic capabilities. These additional functionalities are achieved by incorporating Tb or Gd to the bacteria by culturing them in Tb/Gd supplemented media. The incorporation of Tb provides luminescence properties, enabling potential applications of bacteria as biomarkers. The incorporation of Gd turns bacteria into dual contrast agents for magnetic resonance imaging, since Gd adds T1 contrast to the existing T2 contrast of unmodified bacteria. Given their potential clinical applications, the diagnostic ability of the modified MSR-1 has been successfully tested in vitro in two cell models, confirming their suitability as fluorescent markers (Tb-MSR-1) and dual contrast agents for MR1 (Gd-MSR-1).	May 2023

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