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Instruments / Technical Area	Publication Details	Published	Actions
I13-2-Diamond Manchester Imaging	Technical note: Cartilage imaging with sub‐cellular resolution using a laboratory‐based phase‐contrast x‐ray microscope Michela Esposito , Alberto Astolfo , Silvia Cipiccia , Charlotte Maughan Jones , Savvas N. Savvides , Joseph D. Ferrara , Marco Endrizzi , Jayesh Dudhia , Alessandro Olivo Medical Physics 373 DOI: 10.1002/mp.16599 Diamond Proposal Number(s): [31421] Open Access Show Abstract ▼ Abstract: Background: Microscopic imaging of cartilage is a key tool for the study and development of treatments for osteoarthritis. When cellular and sub-cellular resolution is required, histology remains the gold standard approach, albeit limited by the lack of volumetric information as well as by processing artifacts. Cartilage imaging with the sub-cellular resolution has only been demonstrated in the synchrotron environment. Purpose: To provide a proof-of-concept demonstration of the capability of a laboratory-based x-ray phase-contrast microscope to resolve sub-cellular features in a cartilage sample. Methods: This work is based on a laboratory-based x-ray microscope using intensity-modulation masks. The structured nature of the beam, resulting from the mask apertures, allows the retrieval of three contrast channels, namely, transmission, refraction and dark-field, with resolution depending only on the mask aperture width. An ex vivo equine cartilage sample was imaged with the x-ray microscope and results were validated with synchrotron tomography and histology. Results: Individual chondrocytes, that is, cells responsible for cartilage formation, could be detected with the laboratory-based microscope. The complementarity of the three retrieved contrast channels allowed the detection of sub-cellular features in the chondrocytes. Conclusions: We provide the first proof-of-concept of imaging cartilage tissue with sub-cellular resolution using a laboratory-based x-ray microscope.	Jul 2023

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I13-2-Diamond Manchester Imaging

Technical note: Cartilage imaging with sub‐cellular resolution using a laboratory‐based phase‐contrast x‐ray microscope

Michela Esposito , Alberto Astolfo , Silvia Cipiccia , Charlotte Maughan Jones , Savvas N. Savvides , Joseph D. Ferrara , Marco Endrizzi , Jayesh Dudhia , Alessandro Olivo

Medical Physics 373

DOI: 10.1002/mp.16599

Diamond Proposal Number(s): [31421]