Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I18-Microfocus Spectroscopy	Elemental and structural characterization of heterotopic ossification during achilles tendon healing provides new insights on the formation process Kunal Sharma , Isabella Silva Barreto , Hector Dejea , Malin Hammerman , Christian Appel , Kalotina Geraki , Pernilla Eliasson , Maria Pierantoni , Hanna Isaksson Acs Biomaterials Science & Engineering DOI: 10.1021/acsbiomaterials.4c00935 Diamond Proposal Number(s): [30196] Open Access Show Abstract ▼ Abstract: Heterotopic ossification (HO) in tendons can lead to increased pain and poor tendon function. Although it is believed to share some characteristics with bone, the structural and elemental compositions of HO deposits have not been fully elucidated. This study utilizes a multimodal and multiscale approach for structural and elemental characterization of HO deposits in healing rat Achilles tendons at 3, 6, 12, 16, and 20 weeks post transection. The microscale tomography and scanning electron microscopy results indicate increased mineral density and Ca/P ratio in the maturing HO deposits (12 and 20 weeks), when compared to the early time points (3 weeks). Visually, the mature HO deposits present microstructures similar to calcaneal bone. Through synchrotron-based X-ray scattering and fluorescence, the hydroxyapatite (HA) crystallites are shorter along the c-axis and become larger in the ab-plane with increasing healing time, while the HA crystal thickness remains within the reference values for bone. At the mineralization boundary, the overlap between high levels of calcium and prominent crystallite formation was outlined by the presence of zinc and iron. In the mature HO deposits, the calcium content was highest, and zinc was more present internally, which could be indicative of HO deposit remodeling. This study emphasizes the structural and elemental similarities between the calcaneal bone and HO deposits.	Jul 2024

Instruments / Technical Area

Publication Details

Published

Actions

I18-Microfocus Spectroscopy

Elemental and structural characterization of heterotopic ossification during achilles tendon healing provides new insights on the formation process

Kunal Sharma , Isabella Silva Barreto , Hector Dejea , Malin Hammerman , Christian Appel , Kalotina Geraki , Pernilla Eliasson , Maria Pierantoni , Hanna Isaksson

Acs Biomaterials Science & Engineering

DOI: 10.1021/acsbiomaterials.4c00935

Diamond Proposal Number(s): [30196]