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Instruments / Technical Area	Publication Details	Published	Actions
Krios IV-Titan Krios IV at Diamond Scios-Scios at Diamond	Intracellular morphogenesis of diatom silica is guided by local variations in membrane curvature Lior Aram , Diede De Haan , Neta Varsano , James B. Gilchrist , Christoph Heintze , Ron Rotkopf , Katya Rechav , Nadav Elad , Nils Kröger , Assaf Gal Nature Communications 15 DOI: 10.1038/s41467-024-52211-x Diamond Proposal Number(s): [29609] Open Access Show Abstract ▼ Abstract: Silica cell-wall formation in diatoms is a showcase for the ability of organisms to control inorganic mineralization. The process of silicification by these unicellular algae is tightly regulated within a membrane-bound organelle, the silica deposition vesicle (SDV). Two opposing scenarios were proposed to explain the tight regulation of this intracellular process: a template-mediated process that relies on preformed scaffolds, or a template-independent self-assembly process. The present work points to a third scenario, where the SDV membrane is a dynamic mold that shapes the forming silica. We use in-cell cryo-electron tomography to visualize the silicification process in situ, in its native-state, and with a nanometer-scale resolution. This reveals that the plasma membrane interacts with the SDV membrane via physical tethering at membrane contact sites, where the curvature of the tethered side of the SDV membrane mirrors the intricate silica topography. We propose that silica growth and morphogenesis result from the biophysical properties of the SDV and plasma membranes.	Sep 2024

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Krios IV-Titan Krios IV at Diamond
Scios-Scios at Diamond

Intracellular morphogenesis of diatom silica is guided by local variations in membrane curvature

Lior Aram , Diede De Haan , Neta Varsano , James B. Gilchrist , Christoph Heintze , Ron Rotkopf , Katya Rechav , Nadav Elad , Nils Kröger , Assaf Gal

Nature Communications 15

DOI: 10.1038/s41467-024-52211-x

Diamond Proposal Number(s): [29609]