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Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS	Mutations in the N-domain of aryl hydrocarbon receptor interacting protein affect interactions with heat shock protein 90β and phosphodiesterase 4A5 Marita Vella , Iain W. Manfield , Brandon C. Seychell , Chi H. Trinh , Robert Rambo , G. Nasir Khan , Josanne Vassallo , Therese Hunter , Gary J. Hunter Biochimie 272 DOI: 10.1016/j.biochi.2024.09.005 Diamond Proposal Number(s): [15574, 18536] Open Access Show Abstract ▼ Abstract: The aryl hydrocarbon receptor interacting protein (AIP) is a cytoplasmic molecular co-chaperone and tumour suppressor that assists in protein stability and complex formation involving the aryl hydrocarbon receptor. Germline mutations in the AIP gene predispose to pituitary tumourigenesis with patients exhibiting an aggressive clinical phenotype. Full length AIP harbouring N-domain mutations (R9Q, R16H, V49M and K103R) were purified from E.coli utilizing a methodology that maintained structural integrity and monomeric stability. Mutations did not significantly affect the thermal stability of the protein and caused no overall disruptive effect in the protein structure. The mutations studied lowered the binding affinity of AIP towards two of its binding partners; heat shock protein 90β and phosphodiesterase 4A5 (PDE4A5). The inhibition of phosphodiesterase activity by AIP was also greatly reduced by all mutants. While previously published data has mainly concentrated on the tetratricopeptide repeats of the C-domain of AIP, we present clear evidence that AIP N-domain mutations play a significant role in two protein:protein interactions with partner proteins. The complex interactome of AIP suggests that any observable change in one or more of its binding partners cannot be disregarded as it may have repercussions on other biochemical pathways.	Sep 2024

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B21-High Throughput SAXS

Mutations in the N-domain of aryl hydrocarbon receptor interacting protein affect interactions with heat shock protein 90β and phosphodiesterase 4A5

Marita Vella , Iain W. Manfield , Brandon C. Seychell , Chi H. Trinh , Robert Rambo , G. Nasir Khan , Josanne Vassallo , Therese Hunter , Gary J. Hunter

Biochimie 272

DOI: 10.1016/j.biochi.2024.09.005

Diamond Proposal Number(s): [15574, 18536]