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Instruments / Technical Area	Publication Details	Published	Actions
Krios III-Titan Krios III at Diamond Krios IV-Titan Krios IV at Diamond	Towards a molecular picture of the archaeal cell surface Matthew C. Gaines , Michail N. Isupov , Mathew Mclaren , Clara L. Mollat , Risat U. I. Haque , Jake K. Stephenson , Shamphavi Sivabalasarma , Cyril Hanus , Daniel Kattnig , Vicki A. M. Gold , Sonja Albers , Bertram Daum Nature Communications 15 DOI: 10.1038/s41467-024-53986-9 Diamond Proposal Number(s): [25452, 32707] Open Access Show Abstract ▼ Abstract: Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These include rotary swimming propellers analogous to bacterial flagella (archaella), pili for twitching motility (Aap), adhesive fibres (threads), and filaments facilitating homologous recombination upon UV stress (UV pili). Here, we use cryo-electron microscopy to describe the structure of the S. acidocaldarius archaellum at 2.0 Å resolution, and update the structures of the thread and the Aap pilus at 2.7 Å and 2.6 Å resolution, respectively. We define features unique to archaella of the order Sulfolobales and compare their structure to those of Aap and threads in the context of the S-layer. We define distinct N-glycan patterns in the three filaments and identify a putative O-glycosylation site in the thread. Finally, we ascertain whether N-glycan truncation leads to structural changes in archaella and Aap.	Dec 2024

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Krios III-Titan Krios III at Diamond
Krios IV-Titan Krios IV at Diamond

Towards a molecular picture of the archaeal cell surface