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Instruments / Technical Area	Publication Details	Published	Actions
I04-Macromolecular Crystallography	Synthesis of α-glucan in mycobacteria involves a hetero-octameric complex of trehalose synthase TreS and maltokinase Pep2 Rana Roy , Veeraraghavan Usha , Ali Kermani , David J. Scott , Eva I. Hyde , Gurdyal S. Besra , Luke Alderwick , Klaus Fütterer Acs Chemical Biology 8 (10) DOI: 10.1021/cb400508k PMID: 23901909 Diamond Proposal Number(s): [6388] Open Access Show Abstract ▼ Abstract: Recent evidence established that the cell envelope of Mycobacterium tuberculosis, the bacillus causing tuberculosis (TB), is coated by an α-glucan-containing capsule that has been implicated in persistence in a mouse infection model. As one of three known metabolic routes to α-glucan in mycobacteria, the cytoplasmic GlgE-pathway converts trehalose to α(1 → 4),α(1 → 6)-linked glucan in 4 steps. Whether individual reaction steps, catalyzed by trehalose synthase TreS, maltokinase Pep2, and glycosyltransferases GlgE and GlgB, occur independently or in a coordinated fashion is not known. Here, we report the crystal structure of M. tuberculosis TreS, and show by small-angle X-ray scattering and analytical ultracentrifugation that TreS forms tetramers in solution. Together with Pep2, TreS forms a hetero-octameric complex, and we demonstrate that complex formation markedly accelerates maltokinase activity of Pep2. Thus, complex formation may act as part of a regulatory mechanism of the GlgE pathway, which overall must avoid accumulation of toxic pathway intermediates, such as maltose-1-phosphate, and optimize the use of scarce nutrients.	Jul 2013

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I04-Macromolecular Crystallography

Synthesis of α-glucan in mycobacteria involves a hetero-octameric complex of trehalose synthase TreS and maltokinase Pep2

Rana Roy , Veeraraghavan Usha , Ali Kermani , David J. Scott , Eva I. Hyde , Gurdyal S. Besra , Luke Alderwick , Klaus Fütterer

Acs Chemical Biology 8 (10)

DOI: 10.1021/cb400508k

PMID: 23901909

Diamond Proposal Number(s): [6388]