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Instruments / Technical Area	Publication Details	Published
B21-High Throughput SAXS I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography	The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand N. A. Rodzli , M. P. Lockhart-Cairns , C. W. Levy , J. Chipperfield , L. Bird , C. Baldock , S. M. Prince Biophysical Journal DOI: 10.1016/j.bpj.2020.06.018 Open Access Show Abstract ▼ Abstract: PSD-95 is a member of Membrane Associated Guanylate Kinase class of proteins which form scaffolding interactions with partner proteins including ion and receptor channels. PSD-95 is directly implicated in modulating the electrical responses of excitable cells. The first two PSD-95/Disks Large/Zona Occludens (PDZ) domains of PSD-95 have been shown to be the key component in the formation of channel clusters. We report crystal structures of this dual domain in both in apo- and ligand-bound form; thermodynamic analysis of ligand association and Small Angle X-ray Scattering of the dual domain in the absence and presence of ligands. These experiments reveal that the ligated double domain forms a 3-dimensional scaffold which can be described by a Spacegroup. The concentration of the components in this study is comparable to those found in compartments of excitable cells such as the postsynaptic density and juxta-paranodes of Ranvier. These in vitro experiments inform the basis of the scaffolding function of PSD-95 and provide a detailed model for scaffold formation by the PDZ domains of PSD-95.	Jun 2020
I22-Small angle scattering & Diffraction I24-Microfocus Macromolecular Crystallography	The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions Robert Cleverley , James Kean , Chitra Shintre , Clair Baldock , Jeremy Derrick , Robert C. Ford , Stephen Prince Biochimica Et Biophysica Acta (bba) - Biomembranes DOI: 10.1016/j.bbamem.2015.06.002 Diamond Proposal Number(s): [1023, 1050] Open Access Show Abstract ▼ Abstract: CorA channels are responsible for the uptake of essential magnesium ions by bacteria. X-ray crystal structures have been resolved for two full-length CorA channels, each in a non-conducting state with magnesium ions bound to the protein: These structures reveal a homo-pentameric quaternary structure with approximate 5-fold rotational symmetry about a central pore axis. We report the structure of the detergent solubilized Methanocaldococcus jannaschii CorA channel determined by Cryo-Electron Microscopy and Single Particle Averaging, supported by Small Angle X-ray Scattering and X-ray crystallography. This structure also shows a pentameric channel but with a highly asymmetric domain structure. The asymmetry of the domains includes differential separations between the trans-membrane segments, which reflects mechanical coupling of the cytoplasmic domain to the trans-membrane domain. This structure therefore reveals an important aspect of the gating mechanism of CorA channels by providing an indication of how the absence of magnesium ions leads to major structural changes.	Jun 2015
	Decision making in xia2 Graeme Winter , Carina Lobley , Stephen Prince Acta Crystallographica Section D Biological Crystallography 69, 1260 - 1273 DOI: 10.1107/S0907444913015308 PMID: 23793152 Open Access Show Abstract ▼ Abstract: xia2 is an expert system for the automated reduction of macromolecular crystallography (MX) data employing well trusted existing software. The system can process a full MX data set consisting of one or more sequences of images at one or more wavelengths from images to structure-factor amplitudes with no user input. To achieve this many decisions are made, the rationale for which is described here. In addition, it is critical to support the testing of hypotheses and to allow feedback of results from later stages in the analysis to earlier points where decisions were made: the flexible framework employed by xia2 to support this feedback is summarized here. While the decision-making protocols described here were developed for xia2, they are equally applicable to interactive data reduction.	Jul 2013

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