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Instruments / Technical Area	Publication Details	Published	Actions
B22-Multimode InfraRed imaging And Microspectroscopy	Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy Marie Guilbert , Christophe Eklouh-molinier , Katia Wehbe , Josep Sulé-suso , Ying Yang , Gianfelice Cinque , Pierre Jeannesson , Dhruvananda Sockalingum Journal Of Biomedical Optics 19 (11), 8363-8374 DOI: 10.1117/1.JBO.19.11.111612 PMID: 25193972 Diamond Proposal Number(s): [8422, 8878] Open Access Show Abstract ▼ Abstract: We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation end products (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front.	Nov 2014
B22-Multimode InfraRed imaging And Microspectroscopy	FTIR microspectroscopy of stained cells and tissues. Application in cancer diagnosis Jacek Pijanka , Nick Stone , Gianfelice Cinque , Ying Yang , Achim Kohler , Katia Wehbe , Mark Frogley , Gary Parkes , Joanna Parkes , Paul Dumas , Daniel Van Pittius , Christophe Sandt , Gillian Douce , Dhruvananda Sockalingum , Josep Sulé-suso Spectroscopy 24, 73-78 DOI: 10.3233/SPE-2010-0419 Diamond Proposal Number(s): [2390] Show Abstract ▼ Abstract: It is widely accepted that FTIR spectroscopy has a huge potential in cancer diagnosis. However further work is required to bring this technique into pathology departments. One of the areas where big efforts will be required is the development of cell spectra databases to be used in the diagnosis of cancer. Presently, unstained cytology and tissue samples are studied with FTIR spectroscopy. However, it is not always possible to identify in unstained samples the types of cells present. In order to achieve this, samples need staining after FTIR spectra have been obtained. We have recently shown it is possible to obtain FTIR spectra of stained cells using a synchrotron source (10.1038/labinvest.2010.8). This allows recording FTIR spectra from cells already characterized by pathologists. In order to further this work, we have now obtained FTIR spectra from stained (Papanicolau or Haematoxylin & Eosin) single cells using a benchtop spectrometer. This would be the logical step towards a clinical application in cancer diagnosis. The data here presented show that staining caused a decreased intensity of the peaks at 2920 cm?1 and 2850 cm?1, and the appearance of stronger peaks at 1374 cm?1 and 1040 cm?1. The clinical applications are discussed.	May 2010

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