Search Results

You searched for all publications:

with publication states 'Published (Approved)'

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

Exact matches
Related results

3 items found (0 items not approved), displaying all items.1

Instruments / Technical Area	Publication Details	Published
I24-Microfocus Macromolecular Crystallography	Multiplexing methods in dynamic protein crystallography Margaret A. Klureza , Yelyzaveta Pulnova , David Von Stetten , Robin L. Owen , Godfrey S. Beddard , Arwen R. Pearson , Briony A. Yorke DOI: 10.1016/bs.mie.2024.10.009 Show Abstract ▼ Abstract: Time-resolved X-ray crystallography experiments were first performed in the 1980s, yet they remained a niche technique for decades. With the recent advent of X-ray free electron laser (XFEL) sources and serial crystallographic techniques, time-resolved crystallography has received renewed interest and has become more accessible to a wider user base. Despite this, time-resolved structures represent < 1 % of models deposited in the world-wide Protein Data Bank, indicating that the tools and techniques currently available require further development before such experiments can become truly routine. In this chapter, we demonstrate how applying data multiplexing to time-resolved crystallography can enhance the achievable time resolution at moderately intense monochromatic X-ray sources, ranging from synchrotrons to bench-top sources. We discuss the principles of multiplexing, where this technique may be advantageous, potential pitfalls, and experimental design considerations.	Oct 2024
I24-Microfocus Macromolecular Crystallography	An ultraviolet-driven rescue pathway for oxidative stress to eye lens protein human gamma-D crystallin Jake A. Hill , Yvonne Nyathi , Sam Horrell , David Von Stetten , Danny Axford , Robin L. Owen , Godfrey S. Beddard , Arwen R. Pearson , Helen M. Ginn , Briony Yorke Communications Chemistry 7 DOI: 10.1038/s42004-024-01163-w Diamond Proposal Number(s): [27314] Open Access Show Abstract ▼ Abstract: Human gamma-D crystallin (HGD) is a major constituent of the eye lens. Aggregation of HGD contributes to cataract formation, the leading cause of blindness worldwide. It is unique in its longevity, maintaining its folded and soluble state for 50-60 years. One outstanding question is the structural basis of this longevity despite oxidative aging and environmental stressors including ultraviolet radiation (UV). Here we present crystallographic structures evidencing a UV-induced crystallin redox switch mechanism. The room-temperature serial synchrotron crystallographic (SSX) structure of freshly prepared crystallin mutant (R36S) shows no post-translational modifications. After aging for nine months in the absence of light, a thiol-adduct (dithiothreitol) modifying surface cysteines is observed by low-dose SSX. This is shown to be UV-labile in an acutely light-exposed structure. This suggests a mechanism by which a major source of crystallin damage, UV, may also act as a rescuing factor in a finely balanced redox system.	Apr 2024
I24-Microfocus Macromolecular Crystallography	Time-resolved crystallography using the Hadamard transform Briony Yorke , Robin Owen , Godfrey Beddard , Arwen Pearson Nature Methods DOI: 10.1038/nmeth.3139 PMID: 25282611 Diamond Proposal Number(s): [8367, 5810] Show Abstract ▼ Abstract: We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.	Oct 2014

Publications Database

Search Results

You searched for all publications:

Search Again...

Subject Areas (check all that apply) select all

Instruments used to collect data (check all that apply) select all

Collaborations involved (check all that apply) select all

Diamond Offline Facilities used

Relevant Technical Areas (check all that apply) select all

Menu for Anonymous User