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Instruments / Technical Area	Publication Details	Published	Actions
	Semi-automated microseeding of nanolitre crystallization experiments Thomas Walter , Erika Mancini , Jan Kadlec , Rene Assenberg , Jingshan Ren , Sarah Sainsbury , Ray Owens , Jonathan Grimes , Dave Stuart , Karl Harlos , Stephen Graham Acta Crystallographica Section F Structural Biology And Crystallization Communications 64, 14-18 DOI: 10.1107/S1744309107057260 PMID: 18097093 Show Abstract ▼ Abstract: A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.	Jan 2008

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