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B23-Circular Dichroism	Self-organization of glucose oxidase-polymer surfactant nanoconstructs in solvent-free soft solids and liquids Kamendra Sharma , Yixiong Zhang , Michael R. Thomas , Alex Brogan , Adam Perriman , Stephen Mann The Journal Of Physical Chemistry B 118 (39), 11573 - 11580 DOI: 10.1021/jp507566u Diamond Proposal Number(s): [8467] Show Abstract ▼ Abstract: An anisotropic glucose oxidase–polymer surfactant nanoconjugate is synthesized and shown to exhibit complex temperature-dependent phase behavior in the solvent-free state. At close to room temperature, the nanoconjugate crystallizes as a mesolamellar soft solid with an expanded interlayer spacing of ca. 12 nm and interchain correlation lengths consistent with alkyl tail–tail and PEO–PEO ordering. The soft solid displays a birefringent spherulitic texture and melts at 40 °C to produce a solvent-free liquid protein without loss of enzyme secondary structure. The nanoconjugate melt exhibits a birefringent dendritic texture below the conformation transition temperature (Tc) of glucose oxidase (58 °C) and retains interchain PEO–PEO ordering. Our results indicate that the shape anisotropy of the protein–polymer surfactant globular building block plays a key role in directing mesolamellar formation in the solvent-free solid and suggests that the microstructure observed in the solvent-free liquid protein below Tc is associated with restrictions in the intramolecular motions of the protein core of the nanoconjugate.	Oct 2014

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B23-Circular Dichroism

Self-organization of glucose oxidase-polymer surfactant nanoconstructs in solvent-free soft solids and liquids

Kamendra Sharma , Yixiong Zhang , Michael R. Thomas , Alex Brogan , Adam Perriman , Stephen Mann

The Journal Of Physical Chemistry B 118 (39), 11573 - 11580

DOI: 10.1021/jp507566u

Diamond Proposal Number(s): [8467]

Show Abstract ▼

Oct 2014