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Instruments / Technical Area	Publication Details	Published
B23-Circular Dichroism	Gold nanoparticles’ blocking effect on UV-induced damage to human serum albumin Luigi Calzolai , Stefania Laera , Giacomo Ceccone , Douglas Gilliland , Rohanah Hussain , Giuliano Siligardi , Francois Rossi Journal Of Nanoparticle Research 15 (1) DOI: 10.1007/s11051-012-1412-5 Diamond Proposal Number(s): [7968] Show Abstract ▼ Abstract: Ultraviolet radiation can cause the unfolding and destabilization of proteins. By using high energy photons from a synchrotron radiation source, we show that the UV-induced destabilization of human serum albumin (HSA) can be detected and monitored by measuring the circular dichroism spectrum of the protein. The high flux radiation source damages the HSA protein by causing a partial unfolding of the protein and a significant reduction in the amount of its secondary structure. Gold nanoparticles can effectively stop this UV-induced unfolding of HSA caused by synchrotron radiation. These phenomena could offer interesting applications to protect HSA protein from UV-induced damage and provide an alternative method to measure the relative stability of HSA.	Jan 2013
B23-Circular Dichroism	Structure and Stability of Proteins Interacting with Nanoparticles Luigi Calzolai , Stefania Laera , Giacomo Ceccone , Francois Rossi DOI: 10.1021/bk-2012-1120.ch038 Diamond Proposal Number(s): [7993, 7505] Show Abstract ▼ Abstract: The behavior and toxicological properties of nanoparticles (NP) in biological medium depends heavily on their interactions with proteins. In return, the structure, stability and biological properties of the proteins that interact with the nanoparticles are strongly affected by this interaction. Unfortunately, the mechanisms of interaction and their structural consequences are very difficult to analyse. Here we show the use of advanced biophysical techniques to obtain information on the structure and stability of protein-nanoparticle complexes. By using circular dichroism spectroscopy it is possible to detect changes in the secondary structure and stability of proteins upon interaction with nanoparticles. Moreover, by using nuclear magnetic resonance experiments, it is even possible to detect the specific domain of proteins interacting with nanoparticles.	Dec 2012
B23-Circular Dichroism	Measuring protein structure and stability of protein-nanoparticle systems with synchrotron radiation circular dichroism Stefania Laera , Giacomo Ceccone , Francois Rossi , Douglas Gilliland , Rohanah Hussain , Guiliano Siligardi , Luigi Calzolai Nano Letters 11 (10), 4480 - 4484 DOI: 10.1021/nl202909s PMID: 21932791 Diamond Proposal Number(s): [6192] Show Abstract ▼ Abstract: We measure the structural and stability changes of proteins at nanomolar concentration upon interaction with nanoparticles. Using synchrotron radiation circular dichroism (SRCD), we measure a decrease of 6 °C in the thermal unfolding of human serum albumin upon interaction with silver nanoparticles while this does not happen with gold. The use of SRCD allows measuring critical parameters on protein-nanoparticle interactions, and it will provide experimental data on the relative stability of key biological proteins for nanotoxicology.	Oct 2011

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