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Instruments / Technical Area	Publication Details	Published	Actions
I04-Macromolecular Crystallography	Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine Sarah Batson , Cesira De Chiara , Vita Majce , Adrian J. Lloyd , Stanislav Gobec , Dean Rea , Vilmos Fulop , Christopher Thoroughgood , Katie J. Simmons , Christopher G. Dowson , Colin W. G. Fishwick , Luiz Pedro S. De Carvalho , David I. Roper Nature Communications 8 DOI: 10.1038/s41467-017-02118-7 Open Access Show Abstract ▼ Abstract: D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure.	Dec 2017
I02-Macromolecular Crystallography	Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli Sarah Batson , Dean Rea , Vilmos Fulop , David Roper Acta Crystallographica Section F Structural Biology And Crystallization Communications 66 (4), 405-408 DOI: 10.1107/S1744309110003970 PMID: 20383009 Diamond Proposal Number(s): [310] Show Abstract ▼ Abstract: A recombinant form of Escherichia coli DdlB (EcDdlB) has been prepared and cocrystallized with ADP and d-alanyl-d-alanine to represent the ternary complex of EcDdlB. Furthermore, EcDdlB has been cocrystallized under the same conditions with the ligands ATP and d-alanyl-d-alanine, representing the product-inhibited complex. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 53.0, b = 97.6, c = 109.5 A and a = 51.2, b = 97.8, c = 110.1 A, respectively, and both contained two molecules in the asymmetric unit. Complete data sets were collected to 1.5 and 1.4 A resolution, respectively, from single crystals under cryogenic conditions using synchrotron radiation.	Apr 2010

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