Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I03-Macromolecular Crystallography I04-Macromolecular Crystallography	Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis Charlotte H Coles , Nikolaos Mitakidis , Peng Zhang , Jonathan Elegheert , Weixian Lu , Andrew W. Stoker , Terunaga Nakagawa , Ann Marie Craig , E. Yvonne Jones , A. Radu Aricescu Nature Communications 5 DOI: 10.1038/ncomms6209 PMID: 25385546 Diamond Proposal Number(s): [8423] Open Access Show Abstract ▼ Abstract: Receptor protein tyrosine phosphatase sigma (RPTPσ) regulates neuronal extension and acts as a presynaptic nexus for multiple protein and proteoglycan interactions during synaptogenesis. Unknown mechanisms govern the shift in RPTPσ function, from outgrowth promotion to synaptic organization. Here, we report crystallographic, electron microscopic and small-angle X-ray scattering analyses, which reveal sufficient inter-domain flexibility in the RPTPσ extracellular region for interaction with both cis (same cell) and trans (opposite cell) ligands. Crystal structures of RPTPσ bound to its postsynaptic ligand TrkC detail an interaction surface partially overlapping the glycosaminoglycan-binding site. Accordingly, heparan sulphate and heparin oligomers compete with TrkC for RPTPσ binding in vitro and disrupt TrkC-dependent synaptic differentiation in neuronal co-culture assays. We propose that transient RPTPσ ectodomain emergence from the presynaptic proteoglycan layer allows capture by TrkC to form a trans-synaptic complex, the consequent reduction in RPTPσ flexibility potentiating interactions with additional ligands to orchestrate excitatory synapse formation.	Nov 2014

Instruments / Technical Area

Publication Details

Published

Actions

I03-Macromolecular Crystallography
I04-Macromolecular Crystallography

Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis

Charlotte H Coles , Nikolaos Mitakidis , Peng Zhang , Jonathan Elegheert , Weixian Lu , Andrew W. Stoker , Terunaga Nakagawa , Ann Marie Craig , E. Yvonne Jones , A. Radu Aricescu

Nature Communications 5

DOI: 10.1038/ncomms6209

PMID: 25385546

Diamond Proposal Number(s): [8423]