Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I24-Microfocus Macromolecular Crystallography	Gating topology of the proton-coupled oligopeptide symporters Philip W. Fowler , Marcella Orwick-Rydmark , Sebastian Radestock , Nicolae Solcan , Patricia M. Dijkman , Joseph Lyons , Jane Kwok , Martin Caffrey , Anthony Watts , Lucy R. Forrest , Simon Newstead Structure 23 (2), 290 - 301 DOI: 10.1016/j.str.2014.12.012 PMID: 25651061 Diamond Proposal Number(s): [8224, 8518] Open Access Show Abstract ▼ Abstract: Proton-coupled oligopeptide transporters belong to the major facilitator superfamily (MFS) of membrane transporters. Recent crystal structures suggest the MFS fold facilitates transport through rearrangement of their two six-helix bundles around a central ligand binding site; how this is achieved, however, is poorly understood. Using modeling, molecular dynamics, crystallography, functional assays, and site-directed spin labeling combined with double electron-electron resonance (DEER) spectroscopy, we present a detailed study of the transport dynamics of two bacterial oligopeptide transporters, PepTSo and PepTSt. Our results identify several salt bridges that stabilize outward-facing conformations and we show that, for all the current structures of MFS transporters, the first two helices of each of the four inverted-topology repeat units form half of either the periplasmic or cytoplasmic gate and that these function cooperatively in a scissor-like motion to control access to the peptide binding site during transport.	Feb 2015

Instruments / Technical Area

Publication Details

Published

Actions

I24-Microfocus Macromolecular Crystallography

Gating topology of the proton-coupled oligopeptide symporters