Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I07-Surface & interface diffraction	Structure of lipid multilayers via drop casting of aqueous liposome dispersions Beatrice Sironi , Tim Snow , Christian Redeker , Anna Slastanova , Oier Bikondoa , Tom Arnold , Jacob Klein , Wuge H. Briscoe Soft Matter DOI: 10.1039/C6SM00369A Diamond Proposal Number(s): [1990, 13139] Show Abstract ▼ Abstract: Solid supported lipid multilayers have been widely studied as model cell membranes and as a platform for novel materials. Conventionally, they are prepared from drop casting or spin coating of lipids dissolved in organic solvents, and lipid multilayers prepared from aqueous media have not been previously reported, due to extremely low lipid solubility (i.e. cmc ~10-9 M) in water. Here, the preparation of dioleoylphosphatidylcholine (DOPC) multilayers by drop casting aqueous small unilamellar and multilamellar vesicle or liposome (i.e. SUV and MLV) dispersions is reported on different surfaces, including mica, positively charged polyethylenimine (PEI) coated mica, and stearic trimethylammonium iodide (STAI) coated mica which exposes a monolayer of hydrocarbon tails. X-ray reflectivity (XRR) measurements using a unique “bending mica” method confirm the DOPC multilayer structure on all these substrates, and the influence of the substrate surface chemistry on the multilayer structure is discussed. We suggest that DOPC liposomes serve both as a delivery matrix where appreciable lipid concentration in water (~25 mg mL-1 or 14 mM, i.e. >107 cmc) is feasible, and as a structural precursor where the lamellar structure is readily retained on rupture of the vesicles at the solid surface upon solvent evaporation to facilitate rapid multilayer formation. Our results point towards a controlled preparation of ordered lipid multilayers by tailoring the liposome homogeneity and substrate surface properties, potentially offering a simple method for the inclusion of hydrophilic functional additives (e.g. drugs or nanoparticles) into lipid multilayer based hybrid materials.	Mar 2016

Instruments / Technical Area

Publication Details

Published

Actions

I07-Surface & interface diffraction

Structure of lipid multilayers via drop casting of aqueous liposome dispersions

Beatrice Sironi , Tim Snow , Christian Redeker , Anna Slastanova , Oier Bikondoa , Tom Arnold , Jacob Klein , Wuge H. Briscoe

Soft Matter

DOI: 10.1039/C6SM00369A

Diamond Proposal Number(s): [1990, 13139]

Show Abstract ▼

Mar 2016