Search Results

You searched for all publications:

with publication states 'Published (Approved)'

Search Again...

Author Name

Author Affiliation

Author ORCID

Title of Publication

DOI

Data DOI

Type of Publication (check all that apply) select all

Book Chapter

Conference Paper

Diamond Annual Review Highlight

Editors' Note

Journal Paper

Magazine Article

Poster

Report

Thesis

Title of Journal

Title of Conference

Peer Reviewed:

Yes No Either

Title of Magazine

Abstract

Keywords

Tags

Funding

Diamond Proposal Number

ISBN

BookChapter

Open Access:

Yes No Either

Published In or After Help

Published In or Before Help

Added In or After Help

Added In or Before Help

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

Exact matches
Related results

One item found (0 items not approved).1

Instruments / Technical Area	Publication Details	Published	Actions
I24-Microfocus Macromolecular Crystallography	19F-NMR reveals the role of mobile loops in product and inhibitor binding by the São Paulo metallo-β-lactamase Martine I. Abboud , Philip Hinchliffe , Jurgen Brem , Robert Macsics , Inga Pfeffer , Anne Makena , Klaus-Daniel Umland , Anna M. Rydzik , Guo-Bo Li , James Spencer , Timothy D. W. Claridge , Christopher J. Schofield Angewandte Chemie International Edition 5 DOI: 10.1002/anie.201612185 Diamond Proposal Number(s): [8922] Open Access Show Abstract ▼ Abstract: Resistance to β-lactam antibiotics mediated by metallo-β-lactamases (MBLs) is a growing problem. We describe the use of protein-observe 19F-NMR (PrOF NMR) to study the dynamics of the São Paulo MBL (SPM-1) from β-lactam-resistant Pseudomonas aeruginosa. Cysteinyl variants on the α3 and L3 regions, which flank the di-ZnII active site, were selectively 19F-labeled using 3-bromo-1,1,1-trifluoroacetone. The PrOF NMR results reveal roles for the mobile α3 and L3 regions in the binding of both inhibitors and hydrolyzed β-lactam products to SPM-1. These results have implications for the mechanisms and inhibition of MBLs by β-lactams and non-β-lactams and illustrate the utility of PrOF NMR for efficiently analyzing metal chelation, identifying new binding modes, and studying protein binding from a mixture of equilibrating isomers.	Mar 2017

Publications Database

Search Results

You searched for all publications:

Search Again...

Subject Areas (check all that apply) select all

Instruments used to collect data (check all that apply) select all

Collaborations involved (check all that apply) select all

Diamond Offline Facilities used

Relevant Technical Areas (check all that apply) select all

Menu for Anonymous User