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Instruments / Technical Area	Publication Details	Published	Actions
I02-Macromolecular Crystallography I03-Macromolecular Crystallography I04-Macromolecular Crystallography	Structure of the cohesin loader Scc2 William C. H. Chao , Yasuto Murayama , Sofía Muñoz , Andrew W. Jones , Benjamin O. Wade , Andrew G. Purkiss , Xiao-Wen Hu , Aaron Borg , Ambrosius P. Snijders , Frank Uhlmann , Martin R. Singleton Nature Communications 8 DOI: 10.1038/ncomms13952 Diamond Proposal Number(s): [13775] Open Access Show Abstract ▼ Abstract: The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2–Scc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large ‘hook’ structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2–Scc4 complex. Finally, using recombinantly purified Scc2–Scc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2–Scc4 uses its modular structure to make multiple contacts with cohesin.	Jan 2017

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