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Instruments / Technical Area	Publication Details	Published	Actions
I03-Macromolecular Crystallography I04-Macromolecular Crystallography	An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation Yuzy Matsuo , Sebastian P. Maurer , Masashi Yukawa , Silva Zakian , Martin R. Singleton , Thomas Surrey , Takashi Toda Journal Of Cell Science DOI: 10.1242/jcs.197533 Diamond Proposal Number(s): [13775] Open Access Show Abstract ▼ Abstract: Dynamic microtubule plus-ends interact with various intracellular target regions such as the cell cortex and the kinetochore. Two conserved families of microtubule plus-end-tracking proteins, the XMAP215, ch-TOG or CKAP5 family and the end-binding 1 (EB1, also known as MAPRE1) family, play pivotal roles in regulating microtubule dynamics. Here, we study the functional interplay between fission yeast Dis1, a member of the XMAP215/TOG family, and Mal3, an EB1 protein. Using an in vitro microscopy assay, we find that purified Dis1 autonomously tracks growing microtubule ends and is a bona fide microtubule polymerase. Mal3 recruits additional Dis1 to microtubule ends, explaining the synergistic enhancement of microtubule dynamicity by these proteins. A non-canonical binding motif in Dis1 mediates the interaction with Mal3. X-ray crystallography shows that this new motif interacts in an unconventional configuration with the conserved hydrophobic cavity formed within the Mal3 C-terminal region that typically interacts with the canonical SXIP motif. Selectively perturbing the Mal3–Dis1 interaction in living cells demonstrates that it is important for accurate chromosome segregation. Whereas, in some metazoans, the interaction between EB1 and the XMAP215/TOG family members requires an additional binding partner, fission yeast relies on a direct interaction, indicating evolutionary plasticity of this critical interaction module.	Nov 2016

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I03-Macromolecular Crystallography
I04-Macromolecular Crystallography

An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation

Yuzy Matsuo , Sebastian P. Maurer , Masashi Yukawa , Silva Zakian , Martin R. Singleton , Thomas Surrey , Takashi Toda

Journal Of Cell Science

DOI: 10.1242/jcs.197533

Diamond Proposal Number(s): [13775]