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Krios I-Titan Krios I at Diamond	Architecture of eukaryotic mRNA 3′-end processing machinery Ana Casanal , Ananthanarayanan Kumar , Chris H. Hill , Ashley D. Easter , Paul Emsley , Gianluca Degliesposti , Yuliya Gordiyenko , Balaji Santhanam , Jana Wolf , Katrin Wiederhold , Gillian L. Dornan , Mark Skehel , Carol V. Robinson , Lori A. Passmore Science 546 DOI: 10.1126/science.aao6535 Diamond Proposal Number(s): [15622] Open Access Show Abstract ▼ Abstract: Newly transcribed eukaryotic pre-mRNAs are processed at their 3′ ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined a 3.5-angstrom-resolution structure of the ~200-kilodalton polymerase module. This revealed four beta-propellers, in an assembly markedly similar to those of other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate messenger RNA 3′-end processing	Oct 2017

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