Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I24-Microfocus Macromolecular Crystallography	The C-terminal extension landscape of naturally presented HLA-I ligands Philippe Guillaume , Sarah Picaud , Petra Baumgaertner , Nicole Montandon , Julien Schmidt , Daniel E. Speiser , George Coukos , Michal Bassani-Sternberg , Panagis Filippakopoulos , David Gfeller Proceedings Of The National Academy Of Sciences 115, 5083 - 5088 DOI: 10.1073/pnas.1717277115 Diamond Proposal Number(s): [15433] Show Abstract ▼ Abstract: HLA-I molecules play a central role in antigen presentation. They typically bind 9- to 12-mer peptides, and their canonical binding mode involves anchor residues at the second and last positions of their ligands. To investigate potential noncanonical binding modes, we collected in-depth and accurate HLA peptidomics datasets covering 54 HLA-I alleles and developed algorithms to analyze these data. Our results reveal frequent (442 unique peptides) and statistically significant C-terminal extensions for at least eight alleles, including the common HLA-A03:01, HLA-A31:01, and HLA-A68:01. High resolution crystal structure of HLA-A68:01 with such a ligand uncovers structural changes taking place to accommodate C-terminal extensions and helps unraveling sequence and structural properties predictive of the presence of these extensions. Scanning viral proteomes with the C-terminal extension motifs identifies many putative epitopes and we demonstrate direct recognition by human CD8+ T cells of a 10-mer epitope from cytomegalovirus predicted to follow the C-terminal extension binding mode.	May 2018

Instruments / Technical Area

Publication Details

Published

Actions

I24-Microfocus Macromolecular Crystallography

The C-terminal extension landscape of naturally presented HLA-I ligands

Philippe Guillaume , Sarah Picaud , Petra Baumgaertner , Nicole Montandon , Julien Schmidt , Daniel E. Speiser , George Coukos , Michal Bassani-Sternberg , Panagis Filippakopoulos , David Gfeller

Proceedings Of The National Academy Of Sciences 115, 5083 - 5088

DOI: 10.1073/pnas.1717277115

Diamond Proposal Number(s): [15433]

Show Abstract ▼

May 2018