Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I03-Macromolecular Crystallography I04-Macromolecular Crystallography	Visualization of membrane protein crystals in lipid cubic phase using X-ray imaging Wes Armour , Danny Axford , Mark Basham , Thomas Connolley , David R. Hall , Sam Horrell , Katherine Mcauley , Vitaliy Mykhaylyk , Armin Wagner , Gwyndaf Evans , Anna Warren Acta Crystallographica Section D Biological Crystallography 69, 1252 - 1259 DOI: 10.1107/S0907444913011359 PMID: 23793151 Open Access Show Abstract ▼ Abstract: The focus in macromolecular crystallography is moving towards even more challenging target proteins that often crystallize on much smaller scales and are frequently mounted in opaque or highly refractive materials. It is therefore essential that X-ray beamline technology develops in parallel to accommodate such difficult samples. In this paper, the use of X-ray microradiography and microtomography is reported as a tool for crystal visualization, location and characterization on the macromolecular crystallography beamlines at the Diamond Light Source. The technique is particularly useful for microcrystals and for crystals mounted in opaque materials such as lipid cubic phase. X-ray diffraction raster scanning can be used in combination with radiography to allow informed decision-making at the beamline prior to diffraction data collection. It is demonstrated that the X-ray dose required for a full tomography measurement is similar to that for a diffraction grid-scan, but for sample location and shape estimation alone just a few radiographic projections may be required.	Jul 2013

Instruments / Technical Area

Publication Details

Published

Actions

I03-Macromolecular Crystallography
I04-Macromolecular Crystallography

Visualization of membrane protein crystals in lipid cubic phase using X-ray imaging