Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I04-1-Macromolecular Crystallography (fixed wavelength)	Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs Juliana Roverta Torini , Adriano De Freitas Fernandes , Vitor Hugo Balasco Serrao , Larissa Romanello , Louise Bird , Joanne E. Nettleship , Raymond J. Owens , Jose Brandao-Neto , Ana Eliza Zeraik , Ricardo Demarco , Humberto D'Muniz Pereira Molecular And Biochemical Parasitology 231 DOI: 10.1016/j.molbiopara.2019.111187 Diamond Proposal Number(s): [5073, 14493] Show Abstract ▼ Abstract: Nucleoside diphosphate kinases (NDPKs) are crucial to keep the high triphosphate nucleotide levels in the biological process. The enzymatic mechanism has been extensively described; however, the structural characteristics and kinetic parameters have never been fully determined. In Schistosoma mansoni, NDPK (SmNDPK) is directly involved in the pyrimidine and purine salvage pathways, being essential for nucleotide metabolism. The SmNDPK enzymatic activity is the highest of the known purine metabolisms when compared to the mammalian NDPKs, suggesting the importance of this enzyme in the worm metabolism. Here, we report the recombinant expression of SmNDPK that resulted in 1.7 and 1.9 Å apo-form structure in different space-groups, as well as the 2.1 Å SmNDPK.ADP complex. The binding and kinetic assays reveal the ATP-dependence for enzyme activation. Moreover, in situ hybridization showed that SmNDPK transcripts are found in reproductive organs and in the esophagus gland of adult worms, which can be intrinsically related with the oviposition and digestive processes. These results will help us fully understand the crucial participation of this enzyme in Schistosoma mansoni and its importance for the pathology of the disease.	Jul 2019

Instruments / Technical Area

Publication Details

Published

Actions

I04-1-Macromolecular Crystallography (fixed wavelength)

Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs