Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
B23-Circular Dichroism	The Tim-3-Galectin-9 pathway and its regulatory mechanisms in human breast cancer Inna M. Yasinska , Svetlana S. Sakhnevych , Ludmila Pavlova , Anette Teo Hansen Selnø , Ana Maria Teuscher Abeleira , Ouafa Benlaouer , Isabel Gonçalves Silva , Marianne Mosimann , Luca Varani , Marco Bardelli , Rohanah Hussain , Giuliano Siligardi , Dietmar Cholewa , Steffen M. Berger , Bernhard F. Gibbs , Yuri A. Ushkaryov , Elizaveta Fasler-Kan , Elena Klenova , Vadim V. Sumbayev Frontiers In Immunology 10 DOI: 10.3389/fimmu.2019.01594 Diamond Proposal Number(s): [20755] Open Access Show Abstract ▼ Abstract: Human cancer cells operate a variety of effective molecular and signaling mechanisms which allow them to escape host immune surveillance and thus progress the disease. We have recently reported that the immune receptor Tim-3 and its natural ligand galectin-9 are involved in the immune escape of human acute myeloid leukemia (AML) cells. These cells use the neuronal receptor latrophilin 1 (LPHN1) and its ligand fibronectin leucine rich transmembrane protein 3 (FLRT3, and possibly other ligands) to trigger the pathway. We hypothesized that the Tim-3-galectin-9 pathway may be involved in the immune escape of cancer cells of different origins. We found that studied breast tumors expressed significantly higher levels of both galectin-9 and Tim-3 compared to healthy breast tissues of the same patients and that these proteins were co-localized. Increased levels of LPHN2 and expressions of LPHN3 as well as FLRT3 were also detected in breast tumor cells. Activation of this pathway facilitated the translocation of galectin-9 onto the tumor cell surface, however no secretion of galectin-9 by tumor cells was observed. Surface-based galectin-9 was able to protect breast carcinoma cells against cytotoxic T cell-induced death. Furthermore, we found that cell lines from brain, colorectal, kidney, blood/mast cell, liver, prostate, lung, and skin cancers expressed detectable amounts of both Tim-3 and galectin-9 proteins. The majority of cell lines expressed one of the LPHN isoforms and FLRT3. We conclude that the Tim-3-galectin-9 pathway is operated by a wide range of human cancer cells and is possibly involved in prevention of anti-tumor immunity.	Jul 2019

Instruments / Technical Area

Publication Details

Published

Actions

B23-Circular Dichroism

The Tim-3-Galectin-9 pathway and its regulatory mechanisms in human breast cancer