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Instruments / Technical Area	Publication Details	Published	Actions
I03-Macromolecular Crystallography I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography	Molecular basis of sulfosugar selectivity in sulfoglycolysis Mahima Sharma , Palika Abayakoon , Ruwan Epa , Yi Jin , James P. Lingford , Tomohiro Shimada , Masahiro Nakano , Janice W.-Y. Mui , Akira Ishihama , Ethan D. Goddard-Borger , Gideon J. Davies , Spencer J. Williams Acs Central Science 20 DOI: 10.1021/acscentsci.0c01285 Diamond Proposal Number(s): [13587, 18598, 24948] Open Access Show Abstract ▼ Abstract: The sulfosugar sulfoquinovose (SQ) is produced by essentially all photosynthetic organisms on Earth and is metabolized by bacteria through the process of sulfoglycolysis. The sulfoglycolytic Embden–Meyerhof–Parnas pathway metabolizes SQ to produce dihydroxyacetone phosphate and sulfolactaldehyde and is analogous to the classical Embden–Meyerhof–Parnas glycolysis pathway for the metabolism of glucose-6-phosphate, though the former only provides one C3 fragment to central metabolism, with excretion of the other C3 fragment as dihydroxypropanesulfonate. Here, we report a comprehensive structural and biochemical analysis of the three core steps of sulfoglycolysis catalyzed by SQ isomerase, sulfofructose (SF) kinase, and sulfofructose-1-phosphate (SFP) aldolase. Our data show that despite the superficial similarity of this pathway to glycolysis, the sulfoglycolytic enzymes are specific for SQ metabolites and are not catalytically active on related metabolites from glycolytic pathways. This observation is rationalized by three-dimensional structures of each enzyme, which reveal the presence of conserved sulfonate binding pockets. We show that SQ isomerase acts preferentially on the β-anomer of SQ and reversibly produces both SF and sulforhamnose (SR), a previously unknown sugar that acts as a derepressor for the transcriptional repressor CsqR that regulates SQ-utilization. We also demonstrate that SF kinase is a key regulatory enzyme for the pathway that experiences complex modulation by the metabolites SQ, SLA, AMP, ADP, ATP, F6P, FBP, PEP, DHAP, and citrate, and we show that SFP aldolase reversibly synthesizes SFP. This body of work provides fresh insights into the mechanism, specificity, and regulation of sulfoglycolysis and has important implications for understanding how this biochemistry interfaces with central metabolism in prokaryotes to process this major repository of biogeochemical sulfur.	Feb 2021
I10-Beamline for Advanced Dichroism - scattering	Coherent transfer of spin angular momentum by evanescent spin waves within antiferromagnetic NiO Maciej Dabrowski , Takafumi Nakano , David Burn , Andreas Frisk , David G. Newman , Christoph Klewe , Qian Li , Mengmeng Yang , Padraic Shafer , Elke Arenholz , Thorsten Hesjedal , Gerrit Van Der Laan , Zi Q. Qiu , Robert J. Hicken Physical Review Letters 124, 217201 DOI: 10.1103/PhysRevLett.124.217201 Diamond Proposal Number(s): [17745, 19116, 20760] Show Abstract ▼ Abstract: Insulating antiferromagnets have recently emerged as efficient and robust conductors of spin current. Element-specific and phase-resolved x-ray ferromagnetic resonance has been used to probe the injection and transmission of ac spin current through thin epitaxial NiO(001) layers. The spin current is found to be mediated by coherent evanescent spin waves of GHz frequency, rather than propagating magnons of THz frequency, paving the way towards coherent control of the phase and amplitude of spin currents within an antiferromagnetic insulator at room temperature.	May 2020

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I03-Macromolecular Crystallography
I04-1-Macromolecular Crystallography (fixed wavelength)
I04-Macromolecular Crystallography

Molecular basis of sulfosugar selectivity in sulfoglycolysis