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287 items found (0 items not approved), displaying 1 to 10.[First/Prev] 1, 2, 3, 4, 5, 6, 7, 8 [Next/Last]

Instruments / Technical Area	Publication Details	Published
B21-High Throughput SAXS I04-Macromolecular Crystallography	A structural basis for chaperone repression of stress signaling from the endoplasmic reticulum Lisa Neidhardt , Joanne Tung , Miriam Kuchersky , Jakub Milczarek , Vasileios Kargas , Katherine Stott , Rina Rosenzweig , David Ron , Yahui Yan Molecular Cell DOI: 10.1016/j.molcel.2025.09.032 Diamond Proposal Number(s): [28677] Open Access Show Abstract ▼ Abstract: The endoplasmic reticulum (ER) unfolded protein response (UPR) is tuned by the balance between unfolded proteins and chaperones. Reserve chaperones suppress UPR transducers via their stress-sensing luminal domains, but the underlying mechanisms remain unclear. The ER chaperone AGR2 is known to repress the UPR transducer IRE1β. Here, structural prediction, X-ray crystallography, and NMR spectroscopy identify critical interactions between an AGR2 monomer and a regulatory loop in IRE1β’s luminal domain. However, in the repressive complex, it is an AGR2 dimer that binds IRE1β. Cryoelectron microscopy (cryo-EM) reconstruction explains this feature: one AGR2 protomer engages the regulatory loop, while the second asymmetrically binds IRE1β’s luminal domain’s C terminus, blocking IRE1β-activating dimerization. Molecular dynamic simulations indicate that the second, disruptive AGR2 protomer exploits rare fluctuations in the IRE1β dimer that expose its binding site. Thus, AGR2 disrupts IRE1β dimers to suppress the UPR, priming the system for activation by chaperone clients that compete for AGR2.	Oct 2025
B18-Core EXAFS I14-Hard X-ray Nanoprobe I18-Microfocus Spectroscopy I19-Small Molecule Single Crystal Diffraction	Synchrotrons, neutron sources, and XFELs guiding the future of safe and sustainable nanomaterials Swaroop Chakraborty , Sylvia Britto , Miguel Gomez-Gonzalez , Ana Guilherme Buzanich , Iuliia Mikulska Cell Reports Physical Science 8 DOI: 10.1016/j.xcrp.2025.102806 Diamond Proposal Number(s): [33674, 35117, 35776, 40942, 37789] Open Access Show Abstract ▼ Abstract: Achieving safe and sustainable nanomaterials remains challenging—not necessarily from limited synthetic innovation but due to gaps in observing structural and chemical transformations under environmental conditions. Here, we make a call for a tri-beam operando characterization strategy, integrating synchrotron, neutron, and X-ray free-electron laser (XFEL) techniques into one synergistic experimental framework. Unlike traditional methods providing disconnected snapshots, tri-beam analysis dynamically tracks nanomaterial evolution from atomic-scale changes to structural collapse under near-real-world/quasi-realistic conditions. This holistic approach reveals previously hidden degradation pathways, transient states, and physicochemical thresholds that reshape definitions of material safety. Enhanced by robotic automation, machine learning, and findable, accessible, interoperable, and reusable (FAIR) data principles, our method directly supports Europe’s safe and sustainable by design (SSbD) initiative. We propose embedding tri-beam datasets into regulatory standards, predictive models, and AI-driven screening workflows. Ultimately, tri-beam operando characterization represents a transformative platform for designing resilient, high-performance nanomaterials that meet the environmental and societal demands of the 21st century.	Sep 2025
B21-High Throughput SAXS	A substrate-interacting region of Parkin directs ubiquitination of the mitochondrial GTPase Miro1 Joanna Koszela , Anne Rintala-Dempsey , Giulia Salzano , Viveka Pimenta , Outi Kamarainen , Mads Gabrielsen , Aasna L. Parui , Gary S. Shaw , Helen Walden Journal Of Cell Biology 224 DOI: 10.1083/jcb.202408025 Diamond Proposal Number(s): [28516] Show Abstract ▼ Abstract: Mutations in the E3 ubiquitin ligase Parkin gene have been linked to early onset Parkinson’s disease. Besides many other roles, Parkin is involved in clearance of damaged mitochondria via mitophagy—a process of particular importance in dopaminergic neurons. Upon mitochondrial damage, Parkin accumulates at the outer mitochondrial membrane and is activated, leading to ubiquitination of many mitochondrial substrates and recruitment of mitophagy effectors. While the activation mechanisms of autoinhibited Parkin have been extensively studied, it remains unknown how Parkin recognizes its substrates for ubiquitination. Here, we characterize a conserved region in the flexible linker between the Ubl and RING0 domains of Parkin, which is indispensable for Parkin interaction with the mitochondrial GTPase Miro1. Our results may explain fast kinetics of Miro1 ubiquitination by Parkin in recombinant assays and provide a biochemical explanation for Miro1-dependent Parkin recruitment to the mitochondrial membrane observed in cells. Our findings are important for understanding mitochondrial homeostasis and may inspire new therapeutic avenues for Parkinson’s disease.	Aug 2025
DIAD-Dual Imaging and Diffraction Beamline I13-2-Diamond Manchester Imaging	Variations in mineral prestrain, nanostructure, and microarchitecture play a role in intervertebral disc loading Alissa Parmenter , Elis Newham , Aikta Sharma , Catherine M. Disney , Hans Deyhle , Federico Bosi , Nick J. Terrill , Brian K. Bay , Andrew A. Pitsillides , Himadri S. Gupta , Peter Lee Cell Biomaterials 5 DOI: 10.1016/j.celbio.2025.100151 Diamond Proposal Number(s): [29633, 29784] Open Access Show Abstract ▼ Abstract: The function of all musculoskeletal joints depends on hierarchical structures spanning the molecular to whole-joint scales. Investigating biomechanics across length scales requires correlative multiscale experimental methods. This study applies multimodal in situ synchrotron imaging techniques to spinal joints—focusing on the vertebral endplates—to explore relationships between structure and mechanical strain across spatial scales. Strain mapping using digital volume correlation combined with microarchitectural analysis reveals that high tensile and shear strains play a role in the cartilage to bone transition. Correlative imaging and diffraction show that bone contains narrower mineral nanocrystallites under greater compressive prestrain compared with calcified cartilage. We hypothesize that this multiscale structural adaptation supports the mechanical function of the intervertebral disc. Future applications of the techniques presented here have potential to help unravel the biomechanical underpinnings of pathologies affecting mineralized tissue structure. The multiscale structure-function relationships uncovered here may inspire the design of biomaterials and orthopedic implants.	Jul 2025
Krios III-Titan Krios III at Diamond	Structures of the human adult muscle-type nicotinic receptor in resting and desensitized states Anna Li , Ashley C. W. Pike , Richard Webster , Susan Maxwell , Wei-Wei Liu , Gamma Chi , Jacqueline Palace , David Beeson , David B. Sauer , Yin Yao Dong Cell Reports 44 DOI: 10.1016/j.celrep.2025.115581 Diamond Proposal Number(s): [28713] Open Access Show Abstract ▼ Abstract: Muscle-type nicotinic acetylcholine receptor (AChR) is the key signaling molecule in neuromuscular junctions. Here, we present the structures of full-length human adult receptors in complex with Fab35 in α-bungarotoxin (αBuTx)-bound resting states and ACh-bound desensitized states. In addition to identifying the conformational changes during recovery from desensitization, we also used electrophysiology to probe the effects of eight previously unstudied AChR genetic variants found in patients with congenital myasthenic syndrome (CMS), revealing they cause either slow- or fast-channel CMS characterized by prolonged or abbreviated ion channel bursts. The combined kinetic and structural data offer a better understanding of both the AChR state transition and the pathogenic mechanisms of disease variants.	May 2025
	Tethering ferredoxin-NADP+ reductase to photosystem I promotes photosynthetic cyclic electron transfer Thomas Z. Emrich-Mills , Matthew S. Proctor , Gustaf E. Degen , Philip J. Jackson , Katherine H. Richardson , Frederick R. Hawkings , Felix Buchert , Andrew Hitchcock , C. Neil Hunter , Luke C. M. Mackinder , Michael Hippler , Matthew P. Johnson The Plant Cell DOI: 10.1093/plcell/koaf042 Open Access Show Abstract ▼ Abstract: Fixing CO2 via photosynthesis requires ATP and NADPH, which can be generated through linear electron transfer (LET). However, depending on the environmental conditions, additional ATP may be required to fix CO2, which can be generated by cyclic electron transfer (CET). How the balance between LET and CET is determined remains largely unknown. Ferredoxin-NADP+ reductase (FNR) may act as the switch between LET and CET, channelling photosynthetic electrons to LET when it is bound to photosystem I (PSI) or to CET when it is bound to cytochrome b6f. The essential role of FNR in LET precludes the use of a direct gene knock-out to test this hypothesis. Nevertheless, we circumvented this problem using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-mediated gene editing in Chlamydomonas reinhardtii. Through this approach, we created a chimeric form of FNR tethered to PSI via PSAF. Chimeric FNR mutants exhibited impaired photosynthetic growth and LET along with enhanced PSI acceptor side limitation relative to the wild type due to slower NADPH reduction. However, the chimeric FNR mutants also showed enhanced ΔpH production and NPQ resulting from increased CET. Overall, our results suggest that rather than promoting LET, tethering FNR to PSI promotes CET at the expense of LET and CO2 fixation.	Mar 2025
B21-High Throughput SAXS	Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends Saishree S. Iyer , Fangrui Chen , Funso E. Ogunmolu , Shoeib Moradi , Vladimir A. Volkov , Emma J. Van Grinsven , Chris Van Hoorn , Jingchao Wu , Nemo Andrea , Shasha Hua , Kai Jiang , Ioannis Vakonakis , Mia Potočnjak , Franz Herzog , Benoît Gigant , Nikita Gudimchuk , Kelly E. Stecker , Marileen Dogterom , Michel O. Steinmetz , Anna Akhmanova Journal Of Cell Biology 224 DOI: 10.1083/jcb.202406061 Diamond Proposal Number(s): [21035] Open Access Show Abstract ▼ Abstract: Centrioles are microtubule-based organelles required for the formation of centrosomes and cilia. Centriolar microtubules, unlike their cytosolic counterparts, are stable and grow very slowly, but the underlying mechanisms are poorly understood. Here, we reconstituted in vitro the interplay between the proteins that cap distal centriole ends and control their elongation: CP110, CEP97, and CPAP/SAS-4. We found that whereas CEP97 does not bind to microtubules directly, CP110 autonomously binds microtubule plus ends, blocks their growth, and inhibits depolymerization. Cryo-electron tomography revealed that CP110 associates with the luminal side of microtubule plus ends and suppresses protofilament flaring. CP110 directly interacts with CPAP, which acts as a microtubule polymerase that overcomes CP110-induced growth inhibition. Together, the two proteins impose extremely slow processive microtubule growth. Disruption of CP110–CPAP interaction in cells inhibits centriole elongation and increases incidence of centriole defects. Our findings reveal how two centriolar cap proteins with opposing activities regulate microtubule plus-end elongation and explain their antagonistic relationship during centriole formation.	Mar 2025
I19-Small Molecule Single Crystal Diffraction	Encapsulation of hydrophobic pollutants within a large water-soluble [Fe4L6]4− cage Jack D. Wright , George F. S. Whitehead , Edward O. Pyzer-Knapp , Imogen A. Riddell Cell Reports Physical Science 9 DOI: 10.1016/j.xcrp.2025.102404 Diamond Proposal Number(s): [31541] Open Access Show Abstract ▼ Abstract: Hydrophobic molecules lost in water systems can have significant impacts on local ecology. Notably, the discharge of unmetabolized medications, specifically hormones, and hygiene products into effluent wastewater is a pressing environmental challenge. While selective capture of these molecules has been demonstrated by metal-organic cages (MOCs), these studies typically employ an organic solvent system that does not reflect aqueous environmental conditions. In this study, we report a rare, water-soluble, tetrahedral MOC bearing hydrosolvating sulfonate moieties alongside an aromatic naphthyl spacer. The MOC encloses a large (657 Å3) cavity that can bind a range of polycyclic structures in aqueous media. Isothermal titration calorimetry measurements support guest binding being an enthalpically driven hydrophobic binding process. The synthetic strategy employed to prepare the MOC is extensible and thus serves as a promising route to a range of large, water-soluble MOCs containing hydrophobic binding cavities with exciting prospects.	Jan 2025
I03-Macromolecular Crystallography I04-Macromolecular Crystallography	Biochemical and structural characterization of enzymes in the 4-hydroxybenzoate catabolic pathway of lignin-degrading white-rot fungi Eugene Kuatsjah , Alexa Schwartz , Michael Zahn , Konstantinos Tornesakis , Zoe A. Kellermyer , Morgan A. Ingraham , Sean P. Woodworth , Kelsey J. Ramirez , Paul A. Cox , Andrew R. Pickford , Davinia Salvachúa Cell Reports 43 DOI: 10.1016/j.celrep.2024.115002 Diamond Proposal Number(s): [23269] Open Access Show Abstract ▼ Abstract: White-rot fungi (WRF) are the most efficient lignin-degrading organisms in nature. However, their capacity to use lignin-related aromatic compounds, such as 4-hydroxybenzoate, as carbon sources has only been described recently. Previously, the hydroxyquinol pathway was proposed for the bioconversion of these compounds in fungi, but gene- and structure-function relationships of the full enzymatic pathway remain uncharacterized in any single fungal species. Here, we characterize seven enzymes from two WRF, Trametes versicolor and Gelatoporia subvermispora, which constitute a four-enzyme cascade from 4-hydroxybenzoate to β-ketoadipate via the hydroxyquinol pathway. Furthermore, we solve the crystal structure of four of these enzymes and identify mechanistic differences with the closest bacterial and fungal structural homologs. Overall, this research expands our understanding of aromatic catabolism by WRF and establishes an alternative strategy for the conversion of lignin-related compounds to the valuable molecule β-ketoadipate, contributing to the development of biological processes for lignin valorization.	Dec 2024
I11-High Resolution Powder Diffraction	High-entropy sulfide argyrodite electrolytes for all-solid-state lithium-sulfur batteries Hua Guo , Junhao Li , Matthew Burton , John Cattermull , Yi Liang , Yvonne Chart , Gregory J. Rees , Jack Aspinall , Mauro Pasta Cell Reports Physical Science 120 DOI: 10.1016/j.xcrp.2024.102228 Diamond Proposal Number(s): [25166] Open Access Show Abstract ▼ Abstract: Solid-state electrolytes (SEs) hold the potential to overcome challenges that hinder the commercialization of lithium-sulfur batteries. However, their limited ionic conductivity makes lithium (Li) ion transport a critical bottleneck in accessing the superior capacity of sulfur. In this study, we investigate high-entropy Cl,Br-co-doped sulfide argyrodites as SEs in Li-sulfur (S) all-solid-state batteries (ASSBs). exhibits an ionic conductivity of 6.9 mS at room temperature with an activation energy of 0.28 eV, thanks to its high configurational entropy. The electrolyte with suitable cathodic stability and high ionic conductivity allows S composite cathodes to deliver an areal capacity over 6 mAh when cycled with Li-In anodes. The reduced porosity of the SE separator and the formation of a passivating interphase with metallic Li enable Li-S ASSBs to cycle without short circuits, achieving an S utilization of over 75% at 0.1 C.	Oct 2024

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