Search Results

You searched for all publications:

with publication states 'Published (Approved)'
published in journal 'Current Opinion In Structural Biology'

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

Exact matches
Related results

13 items found (0 items not approved), displaying 1 to 10.[First/Prev] 1, 2 [Next/Last]

Instruments / Technical Area	Publication Details	Published
	Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes Jonathan A. R. Worrall , Michael A. Hough Current Opinion In Structural Biology 77 DOI: 10.1016/j.sbi.2022.102486 Open Access Show Abstract ▼ Abstract: Enzymes with iron-containing active sites play crucial roles in catalysing a myriad of oxidative reactions essential to aerobic life. Defining the three-dimensional structures of iron enzymes in resting, oxy-bound intermediate and substrate-bound states is particularly challenging, not least because of the extreme susceptibility of the Fe(III) and Fe(IV) redox states to radiation-induced chemistry caused by intense X-ray or electron beams. The availability of novel sources such as X-ray free electron lasers has enabled structures that are effectively free of the effects of radiation-induced chemistry and allows time-resolved structures to be determined. Important to both applications is the ability to obtain in crystallo spectroscopic data to identify the redox state of the iron in any particular structure or timepoint.	Dec 2022
	Studying membrane modulation mechanisms by electron cryo-tomography Davide Zabeo , Karen M. Davies Current Opinion In Structural Biology 77 DOI: 10.1016/j.sbi.2022.102464 Open Access Show Abstract ▼ Abstract: Membrane modulation is a key part of cellular life. Critical to processes like energy production, cell division, trafficking, migration and even pathogen entry, defects in membrane modulation are often associated with diseases. Studying the molecular mechanisms of membrane modulation is challenging due to the highly dynamic nature of the oligomeric assemblies involved, which adopt multiple conformations depending on the precise event they are participating in. With the development of electron cryo-tomography and subtomogram averaging, many of these challenges are being resolved as it is now possible to observe complex macromolecular assemblies inside a cell at nanometre to sub-nanometre resolutions. Here, we review the different ways electron cryo-tomography is being used to help uncover the molecular mechanisms used by cells to shape their membranes.	Dec 2022
	Serial synchrotron and XFEL crystallography for studies of metalloprotein catalysis Michael A. Hough , Robin L. Owen Current Opinion In Structural Biology 71, 232 - 238 DOI: 10.1016/j.sbi.2021.07.007 Open Access Show Abstract ▼ Abstract: An estimated half of all proteins contain a metal, with these being essential for a tremendous variety of biological functions. X-ray crystallography is the major method for obtaining structures at high resolution of these metalloproteins, but there are considerable challenges to obtain intact structures due to the effects of radiation damage. Serial crystallography offers the prospect of determining low-dose synchrotron or effectively damage free XFEL structures at room temperature and enables time-resolved or dose-resolved approaches. Complementary spectroscopic data can validate redox and or ligand states within metalloprotein crystals. In this opinion, we discuss developments in the application of serial crystallographic approaches to metalloproteins and comment on future directions.	Dec 2021
NONE-No attached Diamond beamline	Discovery of allosteric binding sites by crystallographic fragment screening Tobias Krojer , James S. Fraser , Frank Von Delft Current Opinion In Structural Biology 65, 209 - 216 DOI: 10.1016/j.sbi.2020.08.004 Show Abstract ▼ Abstract: Understanding allosteric regulation of proteins is fundamental to our study of protein structure and function. Moreover, allosteric binding pockets have become a major target of drug discovery efforts in recent years. However, even though the function of almost every protein can be influenced by allostery, it remains a challenge to discover, rationalise and validate putative allosteric binding pockets. This review examines how the discovery and analysis of putative allosteric binding sites have been influenced by the availability of centralised facilities for crystallographic fragment screening, along with newly developed computational methods for modelling low occupancy features. We discuss the experimental parameters required for success, and how new methods could influence the field in the future. Finally, we reflect on the general problem of how to translate these findings into actual ligand development programs.	Dec 2020
	Recent results in time resolved serial femtosecond crystallography at XFELs Allen M. Orville Current Opinion In Structural Biology 65, 193 - 208 DOI: 10.1016/j.sbi.2020.08.011 Open Access Show Abstract ▼ Abstract: Time-resolved serial femtosecond crystallography (tr-SFX) methods exploit slurries of crystalline samples that range in size from hundreds of nanometers to a few tens of micrometers, at near-physiological temperature and pressure, to generate atomic resolution models and probe authentic function with the same experiment. ‘Dynamic structural biology’ is often used to encompass the research philosophy and techniques. Reaction cycles for tr-SFX studies are initiated by photons or ligand addition/mixing strategies, wherein the latter are potentially generalizable across enzymology. Thus, dynamic structural biology often creates stop-motion molecular movies of macromolecular function. In metal-dependent systems, complementary spectroscopic information can also be collected from the same samples and X-ray pulses, which provides even more detailed mechanistic insights. These types of experimental data also complement quantum mechanical and classical dynamics numerical calculations. Correlated structural-functional results will yield more detailed mechanistic insights and will likely translate into better drugs and treatments impacting human health, and better catalysis for clean energy and agriculture.	Dec 2020
	Advances in cryo-electron tomography and subtomogram averaging and classification Peijun Zhang Current Opinion In Structural Biology DOI: 10.1016/j.sbi.2019.05.021 Open Access Show Abstract ▼ Abstract: Cryo-electron tomography (cryoET) can provide 3D reconstructions, or tomograms, of pleomorphic objects such as organelles or cells in their close-to-native states. Subtomograms that contain repetitive structures can be further extracted and subjected to averaging and classification to improve resolution, and this process has become an emerging structural biology method referred to as cryoET subtomogram averaging and classification (cryoSTAC). Recent technical advances in cryoSTAC have had a profound impact on many fields in biology. Here, I review recent exciting work on several macromolecular assemblies demonstrating the power of cryoSTAC for in situ structure analysis and discuss challenges and future directions.	Jul 2019
I03-Macromolecular Crystallography	Metallocofactor assembly for [FeFe]-hydrogenases Pedro Dinis , Beata M. Wieckowski , Peter L. Roach Current Opinion In Structural Biology 41, 90 - 97 DOI: 10.1016/j.sbi.2016.06.004 Diamond Proposal Number(s): [8889] Show Abstract ▼ Abstract: Hydrogenases are a potential source of environmentally benign bioenergy, using complex cofactors to catalyze the reversible reduction of protons to form hydrogen. The most active subclass, the [FeFe]-hydrogenases, is dependent on a metallocofactor, the H cluster, that consists of a two iron subcluster ([2Fe]H) bridging to a classical cubane cluster ([4Fe–4S]H). The ligands coordinating to the diiron subcluster include an azadithiolate, three carbon monoxides, and two cyanides. To assemble this complex cofactor, three maturase enzymes, HydG, HydE and HydF are required. The biosynthesis of the diatomic ligands proceeds by an unusual fragmentation mechanism, and structural studies in combination with spectroscopic analysis have started to provide insights into the HydG mediated assembly of a [2Fe]H subcluster precursor.	Dec 2016
I02-Macromolecular Crystallography I03-Macromolecular Crystallography I04-Macromolecular Crystallography	The structural biology of patellamide biosynthesis Jesko Koehnke , Andrew Bent , Wael E Houssen , Greg Mann , Marcel Jaspars , James Naismith Current Opinion In Structural Biology 29, 112 - 121 DOI: 10.1016/j.sbi.2014.10.006 Diamond Proposal Number(s): [8268] Open Access Show Abstract ▼ Abstract: The biosynthetic pathways for patellamide and related natural products have recently been studied by structural biology. These pathways produce molecules that have a complex framework and exhibit a diverse array of activity due to the variability of the amino acids that are found in them. As these molecules are difficult to synthesize chemically, exploitation of their properties has been modest. The patellamide pathway involves amino acid heterocyclization, peptide cleavage, peptide macrocyclization, heterocycle oxidation and epimerization; closely related products are also prenylated. Enzyme activities have been identified for all these transformations except epimerization, which may be spontaneous. This review highlights the recent structural and mechanistic work on amino acid heterocyclization, peptide cleavage and peptide macrocyclization. This work should help in using the enzymes to produce novel analogs of the natural products enabling an exploitation of their properties.	Dec 2014
	Micro-crystallography comes of age Janet L. Smith , Robert F. Fischetti , Masaki Yamamoto Current Opinion In Structural Biology 22 (5), 602 - 612 DOI: 10.1016/j.sbi.2012.09.001 Show Abstract ▼ Abstract: The latest revolution in macromolecular crystallography was incited by the development of dedicated, user friendly, micro-crystallography beam lines. Brilliant X-ray beams of diameter 20 μm or less, now available at most synchrotron sources, enable structure determination from samples that previously were inaccessible. Relative to traditional crystallography, crystals with one or more small dimensions have diffraction patterns with vastly improved signal-to-noise when recorded with an appropriately matched beam size. Structures can be solved from isolated, well diffracting regions within inhomogeneous samples. This review summarizes the technological requirements and approaches to producing micro-beams and how they continue to change the practice of crystallography.	Oct 2012
I02-Macromolecular Crystallography I04-Macromolecular Crystallography	Structural insights into the retroviral DNA integration apparatus Peter Cherepanov , Goedele N. Maertens , Stephen Hare Current Opinion In Structural Biology 21 (2), 249 - 256 DOI: 10.1016/j.sbi.2010.12.005 PMID: 21277766 Diamond Proposal Number(s): [317] Show Abstract ▼ Abstract: Retroviral replication depends on successful integration of the viral genetic material into a host cell chromosome. Virally encoded integrase, an enzyme from the DDE(D) nucleotidyltransferase superfamily, is responsible for the key DNA cutting and joining steps associated with this process. Insights into the structural and mechanistic aspects of integration are directly relevant for the development of antiretroviral drugs. Recent breakthroughs have led to biochemical and structural characterization of the principal integration intermediates revealing the tetramer of integrase that catalyzes insertion of both 3' viral DNA ends into a sharply bent target DNA. This review discusses the mechanism of retroviral DNA integration and the mode of action of HIV-1 integrase strand transfer inhibitors in light of the recent visualization of the prototype foamy virus intasome, target DNA capture and strand transfer complexes.	Apr 2011

Publications Database

Search Results

You searched for all publications:

Search Again...

Subject Areas (check all that apply) select all

Instruments used to collect data (check all that apply) select all

Collaborations involved (check all that apply) select all

Diamond Offline Facilities used

Relevant Technical Areas (check all that apply) select all

Menu for Anonymous User