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Instruments / Technical Area	Publication Details	Published	Actions
B22-Multimode InfraRed imaging And Microspectroscopy	Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy Marie Guilbert , Christophe Eklouh-molinier , Katia Wehbe , Josep Sulé-suso , Ying Yang , Gianfelice Cinque , Pierre Jeannesson , Dhruvananda Sockalingum Journal Of Biomedical Optics 19 (11), 8363-8374 DOI: 10.1117/1.JBO.19.11.111612 PMID: 25193972 Diamond Proposal Number(s): [8422, 8878] Open Access Show Abstract ▼ Abstract: We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation end products (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front.	Nov 2014

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B22-Multimode InfraRed imaging And Microspectroscopy

Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy

Marie Guilbert , Christophe Eklouh-molinier , Katia Wehbe , Josep Sulé-suso , Ying Yang , Gianfelice Cinque , Pierre Jeannesson , Dhruvananda Sockalingum

Journal Of Biomedical Optics 19 (11), 8363-8374

DOI: 10.1117/1.JBO.19.11.111612

PMID: 25193972

Diamond Proposal Number(s): [8422, 8878]