Krios I-Titan Krios I at Diamond
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Ana Raquel
Pereira
,
Silvia
Di Francescantonio
,
Ana
Da Rosa Soares
,
Tianyang
Liu
,
Filomena A.
Carvalho
,
Josie Liane
Ferreira
,
Graciano
Leal
,
Inês
Faleiro
,
Naoko
Kogata
,
Beatrice
Labella
,
Teresinha
Evangelista
,
Nuno C.
Santos
,
Michael
Way
,
Carolyn A.
Moores
,
Edgar R.
Gomes
Diamond Proposal Number(s):
[20287]
Open Access
Abstract: A network of plasma membrane invaginations called t-tubules plays an essential role in controlling calcium release from the endoplasmic reticulum at the triads during muscle contraction. Although the importance of t-tubules for muscle physiology is well established, and abnormalities are found in muscle disorders, the mechanisms that mediate t-tubule growth are unknown. We show that the actomyosin cortex beneath the plasma membrane, regulated by Arp2/3 complexes containing Arpc5, acts as a gatekeeper for the membrane availability during t-tubule growth. Enlarged t-tubules are formed upon disruption of Arpc5, impairing the synchronization between plasma membrane depolarization and calcium release. Knockout of Arpc5 in mouse skeletal muscle results in impaired locomotion and posture. Furthermore, we show that human triadopathy patients and Arpc5 knockout mice accumulate enlarged t-tubules. We propose that cortex-dependent membrane availability affects muscle function, offering a potential pathophysiological mechanism for muscle disorders.
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Sep 2026
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I07-Surface & interface diffraction
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Qichun
Gu
,
Tianjun
Liu
,
Yucheng
Xu
,
Xinjuan
Li
,
Yunzhou
Deng
,
Yang
Lu
,
Youcheng
Zhang
,
Zimu
Wei
,
Xinyu
Bai
,
Capucine
Mamak
,
Yutong
Han
,
Alessandro J.
Mirabelli
,
Weidong
Xu
,
Jian
Mao
,
Caterina
Ducati
,
Henning
Sirringhaus
,
Samuel D.
Stranks
,
Miguel
Anaya
Diamond Proposal Number(s):
[32266]
Open Access
Abstract: Perovskite light-emitting diodes (PeLEDs) are promising low-cost, solution-processable, and color-pure optoelectronic devices for display and lighting applications. However, blue PeLEDs continue to lag their green and red counterparts in terms of luminance and operational lifetime, limiting their practical implementation. The performance disparity primarily arises from charge injection imbalance, which accelerates degradation at the perovskite/hole transport layer (HTL) interface under electrical bias. Here, we design a polymer blend HTL comprising poly(N-vinyl-2,7-difluoro-carbazole) (PVK-F) and poly(bis(4-phenyl)(2,4,6-trimethylphenyl)amine) (PTAA), in which strengthened van der Waals interactions promote denser molecular packing. This optimized microstructure simultaneously enhances hole mobility and wettability, enabling high-quality perovskite film formation. Consequently, PeLEDs emitting at 485 nanometers achieve an external quantum efficiency of 24.1% and luminance exceeding 26,000 candela per square meter. Moreover, the improved hole injection mitigates interfacial degradation, yielding an operational half-lifetime exceeding 700 minutes at an initial luminance of 100 candela per square meter. This work establishes polymer blending as an effective strategy for advancing the performance and stability of blue PeLEDs.
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Aug 2026
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Krios I-Titan Krios I at Diamond
Krios IV-Titan Krios IV at Diamond
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Jose Enrique
Gonzalez-Prada
,
Sulin
Liu
,
Chuhan
Shang
,
Chloe S.
Chernoff
,
Damian P.
Bright
,
Martin
Mortensen
,
Charlotte F.
Jones
,
Stephanie
Nestorow
,
Vikram Babu
Kasaragod
,
Wan-Na
Chen
,
Saad
Hannan
,
Jianchong
Zhou
,
Alexander W. E.
Dunn
,
Asma
Soltani
,
Richard J.
Turner
,
Natasha M.
Duggan
,
Yin
Yuan
,
Ayla A.
Wahid
,
Steven W.
Hardwick
,
Suzanne
Scott
,
Dimitri Y.
Chirgadze
,
Els
Pardon
,
Jan
Steyaert
,
A. Radu
Aricescu
,
Ole
Paulsen
,
David
Belin
,
Trevor G.
Smart
,
Paul S.
Miller
Diamond Proposal Number(s):
[31589, 37678]
Open Access
Abstract: γ-Aminobutyric acid type-A (GABAA) receptors are the principal mediators of inhibitory neurotransmission in the human central nervous system. The α2- and α3-containing subtypes have tightly controlled spatial expression profiles, which influence anxiety, nociception, epilepsy, and autism. α2/α3-Selective small molecules compromise on strength of effect (efficacy) to avoid off-subtype modulation. To break this pharmacological deadlock, we study here a panel of nanobodies (NBs) raised against α2- and α3-containing GABAA receptors. We identify subtype selective silent binders, positive allosteric modulators (PAMs), and inhibitors. Cryo–electron microscopy structures explain the binding modes and molecular mechanisms of action of representative NBs. Modulators exhibit distinct synaptic and extrasynaptic functional profiles in brain slices and neuronal networks and can reduce anxiety in vivo. These selective and efficacious NBs (whether inhibitors or positive modulators) enable strong yet precise pharmacological control of α2/α3-containing subtypes to advance basic research and as potential therapeutic leads to treat neuropsychiatric disorders.
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Jul 2026
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I13-2-Diamond Manchester Imaging
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Louis
Verschuren
,
Vladimir
Matskovsky
,
Matthieu N
Boone
,
Pieter
De Frenne
,
Tom
De Mil
,
Kristof
Haneca
,
Shashidhara
Marathe
,
Charlotte
Pearson
,
Christoph
Rau
,
Ute
Sass-Klaassen
,
Joris
Van Acker
,
Kris
Vandekerkhove
,
Kaz
Wanelik
,
Julia
Weidemüller
,
Valerie
Trouet
,
Jan
Van Den Bulcke
Diamond Proposal Number(s):
[36278]
Open Access
Abstract: Maximum latewood density of conifers is the most widely used annual-resolution summer temperature proxy. Regions with few conifers, however, remain underrepresented in global paleoclimate records. Here, we use x-ray micro–computed tomography (micro-CT) to show that latewood density measurements of European beech (Fagus sylvatica L.) in a temperate lowland forest exhibit a strong summer (May to September) temperature signal (r = 0.73; 1833 to 2022 CE). Complementary wood anatomical analyses using deep learning segmentation reveal that both vessel and fiber anatomy are key drivers of latewood density variability and its temperature sensitivity. By integrating these anatomical responses, x-ray micro-CT–based latewood density measurements generate a robust and temporally stable summer temperature signal. Our results highlight the untapped potential of broad-leaved tree species for density-based climate reconstructions in temperate regions and open previously unidentified avenues for high-resolution paleoclimatology beyond the use of conifers.
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Jul 2026
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I03-Macromolecular Crystallography
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Open Access
Abstract: DNA-functionalized colloidal nanoparticles assemble through flexible, nanoscale DNA hybridization interactions that limit atomic-level structural order. Here, we report a valence-centric strategy that enables DNA-bonded, protein single crystals with unconventional mechanical properties. An octameric enzyme, glutarate L-2-hydroxylase, was site- and number-selectively conjugated with eight self-complementary single-stranded DNA, yielding octavalent molecular bonds. The resulting conjugate assembled into the designed body-centered tetragonal crystals that diffracted to 1.42- to 2.61-angstrom resolution, with contacts mediated by B-form DNA helices spanning 17 to 25 angstroms. Increasing oligonucleotide length induces anisotropic lattice expansion while preserving atomic periodicity, even with partial DNA occupancy. Mechanistic studies suggest that the dynamic motion of unhybridized DNA facilitates crystallization, analogous to fluctuating electron clouds in atomic bonding. Compared with native protein crystals, DNA-hybridized crystals are 23-fold softer. These results challenge the assumption that flexibility is incompatible with structural order and establish a programmable framework for biomolecular crystallization and nanomaterials engineering with atomic precision.
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Jul 2026
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I15-Extreme Conditions
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Diamond Proposal Number(s):
[11665]
Open Access
Abstract: Materials properties depend strongly on chemical composition, i.e., the relative amounts of each chemical element. Changes in composition lead to entirely different chemical arrangements, which vary in complexity from perfectly ordered (i.e., stoichiometric compounds) to completely disordered (i.e., solid solutions). Accurately capturing this range of chemical arrangements remains a major challenge, limiting the predictive accuracy of machine learning potentials (MLPs) in materials modeling. Here, we combine information theory and machine learning to optimize the sampling of chemical motifs and design MLPs that effectively capture the behavior of metallic alloys across their entire compositional and structural landscape. The effectiveness of this approach is demonstrated by predicting the compositional dependence of various materials properties—including stacking-fault energies, short-range order, heat capacities, and phase diagrams—for the AuPt and CuAu binary alloys, the ternary CrCoNi, and the TiTaVW high-entropy alloy. Extensive comparison against experimental data demonstrates the robustness of this approach in enabling materials modeling with high physical fidelity.
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Jun 2026
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B18-Core EXAFS
I11-High Resolution Powder Diffraction
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Diamond Proposal Number(s):
[34129]
Open Access
Abstract: Hybrid materials, which combine inorganic and molecular components, often exhibit structural flexibility that enables unusual functional responses. Among them, Prussian blue analogs (PBAs) are a promising class for post–lithium battery technologies. Here, we show that nonequilibrium transformation processes govern the charge-storage mechanism of a PBA electrode, K2Mn[Fe(CN)6]. Ostensibly, this behavior mirrors that observed in high-rate cycling of conventional cathodes such as LiFePO4 yet arises here for fundamentally different reasons–namely, low elastic moduli and cooperative distortions inherent to the hybrid framework. Using operando x-ray absorption spectroscopy with Metropolis matrix factorization and x-ray diffraction, we show that framework flexibility limits transport kinetics and promotes collective, metastable pathways. Our results not only highlight various directions for PBA cathode optimization but also suggest a broader relevance of nonequilibrium mechanisms for mass transport in hybrid materials beyond PBAs alone.
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Jun 2026
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I04-Macromolecular Crystallography
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Jogi
Madhuprakash
,
Amirali
Toghani
,
Hsuan
Pai
,
Madia
Harvey
,
Adam R.
Bentham
,
Benjamin A.
Seager
,
Enoch Lok Him
Yuen
,
Juan Carlos
De La Concepcion
,
David M.
Lawson
,
Clare E. M.
Stevenson
,
Angel
Vergara-Cruces
,
Lida
Derevnina
,
Tolga O.
Bozkurt
,
Mark J.
Banfield
,
Sophien
Kamoun
,
Mauricio P.
Contreras
Diamond Proposal Number(s):
[25108]
Open Access
Abstract: Pathogens counteract central nodes of NLR immune receptor networks to suppress immunity. However, the mechanisms by which pathogens hijack helper NLR pathways are poorly understood. We show that an effector from the late blight pathogen Phytophthora infestans interacts with the host protein NbTOL9a and a helper NLR to suppress immunity. We solved the crystal structure of the RXLR-LWY effector AVRcap1b in complex with the ENTH domain of NbTOL9a. The structure revealed that, unlike other RXLR-LWY effectors, AVRcap1b has a previously unidentified L-shaped fold that defines a distinct structural family of effectors in the genus Phytophthora. We defined the AVRcap1b/NbTOL9a binding interface and designed effector mutants that do not bind NbTOL9a, impairing immune suppression. This suggests that ENTH binding is required for full virulence activity. Last, we show that AVRcap1b associates specifically with activated NbNRC2 independently of NbTOL9a binding. We propose a model in which the effector interconnects NbNRC2 with the NbTOL9a pathway. Our results illustrate a previously uncharacterized pathogen mechanism to hijack NLR pathways and suppress immunity.
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Jun 2026
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B07-B1-Versatile Soft X-ray beamline: High Throughput ES1
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Open Access
Abstract: Anion exchange membrane water electrolysis (AEMWE) represents a promising technology for green hydrogen production. Although numerous efforts have been devoted to optimize the anode catalysis by structural and chemical modulation, the effectiveness of such strategies in enhancing stability remains limited. Herein, we propose a cerium-induced double-shelled structure formation strategy that modulates the electrode/electrolyte interfacial microenvironment through spatial configuration engineering, effectively suppressing anodic corrosion. Mechanistic studies revealed that the associated nanospace enrichment effect increased the coverage of surface hydroxide ion (OH−) species, thereby enhancing the local alkalinity at the material surface and effectively suppressing ion leaching. The double-shelled cerium dioxide (CeO2)/lanthanum cobaltite (LaCoO3)-10% catalyst demonstrated outstanding performance in the AEMWE device, achieving an industrial-relevant current density of 3 amperes per square centimeter at 1.88 volts. Furthermore, the catalyst exhibited exceptional long-term stability exceeding 2000 hours under simulated industrial conditions. Our findings underscore the importance of engineering the physical spatial configuration to regulate the interfacial microenvironment, offering a strategy to address the corrosion degradation of anode catalysts.
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Jun 2026
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I03-Macromolecular Crystallography
I04-Macromolecular Crystallography
I24-Microfocus Macromolecular Crystallography
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Daming
Zhou
,
Abhay
Kotecha
,
James T.
Kelly
,
Peng-Nien
Huang
,
Yi-Yin
Chen
,
Thomas S.
Walter
,
Helen M. E.
Duyvesteyn
,
Raymond J.
Owens
,
Shu-Yuan
Ho
,
Tzou-Yien
Lin
,
Elizabeth E.
Fry
,
Jingshan
Ren
,
Kuan-Ying A.
Huang
,
David I.
Stuart
Diamond Proposal Number(s):
[14744]
Open Access
Abstract: EV-A71 has been responsible for recent severe HFMD outbreaks. We report structures for 12 potently neutralizing human anti–EV-A71 monoclonal antibody Fabs, alone and complexed with virus. Most recognize the native antigenic state with epitopes that span interfaces, together covering 85% of the capsid surface. The majority (8 of 12) bind the canyon, while the others cluster around the icosahedral two- and threefold axes. Blocking SCARB2 receptor binding likely contributes to neutralization for all, and a subset induces empty particles. A predominant gene family (IGHV4-39) does not dictate a common binding pose. Long CDR-H3 loops are frequently key to binding, especially at the canyon, suggesting that antigenicity data based on antibodies with shorter CDR3s (e.g., murine) may be misleading. This dataset reveals neutralization mechanisms for recently circulating EV-A71 genotypes, which will inform immunotherapies. We demonstrate synergy in vitro between canyon binding and both two- and threefold binding antibodies to increase neutralization potency.
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Jun 2026
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