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Instruments / Technical Area	Publication Details	Published	Actions
B23-Circular Dichroism	Antimicrobial peptide capsids of de novo design Emiliana De Santis , Hasan Alkassem , Baptiste Lamarre , Nilofar Faruqui , Angelo Bella , James E. Noble , Nicola Micale , Santanu Ray , Jonathan R. Burns , Alexander R. Yon , Bart W. Hoogenboom , Maxim G. Ryadnov Nature Communications 8 DOI: 10.1038/s41467-017-02475-3 Diamond Proposal Number(s): [12739] Open Access Show Abstract ▼ Abstract: The spread of bacterial resistance to antibiotics poses the need for antimicrobial discovery. With traditional search paradigms being exhausted, approaches that are altogether different from antibiotics may offer promising and creative solutions. Here, we introduce a de novo peptide topology that—by emulating the virus architecture—assembles into discrete antimicrobial capsids. Using the combination of high-resolution and real-time imaging, we demonstrate that these artificial capsids assemble as 20-nm hollow shells that attack bacterial membranes and upon landing on phospholipid bilayers instantaneously (seconds) convert into rapidly expanding pores causing membrane lysis (minutes). The designed capsids show broad antimicrobial activities, thus executing one primary function—they destroy bacteria on contact.	Dec 2017
B21-High Throughput SAXS B23-Circular Dichroism	A De Novo Virus-Like Topology for Synthetic Virions James Noble , Emiliana De Santis , Jascindra Ravi , Baptiste Lamarre , Valeria Castelletto , Judith Mantell , Santanu Ray , Maxim G. Ryadnov Journal Of The American Chemical Society DOI: 10.1021/jacs.6b05751 Diamond Proposal Number(s): [12321, 10083, 10068] Open Access Show Abstract ▼ Abstract: A de novo topology of virus-like assembly is reported. The design is a trifaceted coiled-coil peptide helix, which self-assembles into ultrasmall, monodisperse, anionic virus-like shells that encapsulate and transfer both RNA and DNA into human cells. Unlike existing artificial systems, these shells share the same physical characteristics of viruses being anionic, nonaggregating, abundant, hollow, and uniform in size, while effectively mediating gene silencing and transgene expression. These are the smallest virus-like structures reported to date, both synthetic and native, with the ability to adapt and transfer small and large nucleic acids. The design thus offers a promising solution for engineering bespoke artificial viruses with desired functions.	Sep 2016

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