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Krios III-Titan Krios III at Diamond	High-light versus low-light: Effects on paired photosystem II supercomplex structural rearrangement in pea plants Alessandro Grinzato , Pascal Albanese , Roberto Marotta , Paolo Swuec , Guido Saracco , Martino Bolognesi , Giuseppe Zanotti , Cristina Pagliano International Journal Of Molecular Sciences 21 DOI: 10.3390/ijms21228643 Diamond Proposal Number(s): [19714] Open Access Show Abstract ▼ Abstract: In plant grana thylakoid membranes Photosystem II (PSII) associates with a variable number of antenna proteins (LHCII) to form different types of supercomplexes (PSII-LHCII), whose organization is dynamically adjusted in response to light cues, with the C2S2 more abundant in high-light and the C2S2M2 in low-light. Paired PSII-LHCII supercomplexes interacting at their stromal surface from adjacent thylakoid membranes were previously suggested to mediate grana stacking. Here, we present the cryo-electron microscopy maps of paired C2S2 and C2S2M2 supercomplexes isolated from pea plants grown in high-light and low-light, respectively. These maps show a different rotational offset between the two supercomplexes in the pair, responsible for modifying their reciprocal interaction and energetic connectivity. This evidence reveals a different way by which paired PSII-LHCII supercomplexes can mediate grana stacking at diverse irradiances. Electrostatic stromal interactions between LHCII trimers almost completely overlapping in the paired C2S2 can be the main determinant by which PSII-LHCII supercomplexes mediate grana stacking in plants grown in high-light, whereas the mutual interaction of stromal N-terminal loops of two facing Lhcb4 subunits in the paired C2S2M2 can fulfil this task in plants grown in low-light. The high-light induced accumulation of the Lhcb4.3 protein in PSII-LHCII supercomplexes has been previously reported. Our cryo-electron microscopy map at 3.8 Å resolution of the C2S2 supercomplex isolated from plants grown in high-light suggests the presence of the Lhcb4.3 protein revealing peculiar structural features of this high-light-specific antenna important for photoprotection.	Nov 2020

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Krios III-Titan Krios III at Diamond

High-light versus low-light: Effects on paired photosystem II supercomplex structural rearrangement in pea plants

Alessandro Grinzato , Pascal Albanese , Roberto Marotta , Paolo Swuec , Guido Saracco , Martino Bolognesi , Giuseppe Zanotti , Cristina Pagliano

International Journal Of Molecular Sciences 21

DOI: 10.3390/ijms21228643

Diamond Proposal Number(s): [19714]