Diamond Publications - Search Results

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I04-Macromolecular Crystallography I23-Long wavelength MX I24-Microfocus Macromolecular Crystallography Krios III-Titan Krios III at Diamond	Structure and assembly of the S-layer in C. difficile Paola Lanzoni-Mangutchi , Oishik Banerji , Jason Wilson , Anna Barwinska-Sendra , Joseph A. Kirk , Filipa Vaz , Shauna O’beirne , Arnaud Basle , Kamel El Omari , Armin Wagner , Neil F. Fairweather , Gillian R. Douce , Per A. Bullough , Robert P. Fagan , Paula Salgado Nature Communications 13 DOI: 10.1038/s41467-022-28196-w Diamond Proposal Number(s): [15523, 18598, 19832] Open Access Show Abstract ▼ Abstract: Many bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface and plays crucial roles in cell physiology. Here, we report the crystal structure of SlpA, the main S-layer protein of the bacterial pathogen Clostridioides difficile, and use electron microscopy to study S-layer organisation and assembly. The SlpA crystal lattice mimics S-layer assembly in the cell, through tiling of triangular prisms above the cell wall, interlocked by distinct ridges facing the environment. Strikingly, the array is very compact, with pores of only ~10 Å in diameter, compared to other S-layers (30–100 Å). The surface-exposed flexible ridges are partially dispensable for overall structure and assembly, although a mutant lacking this region becomes susceptible to lysozyme, an important molecule in host defence. Thus, our work gives insights into S-layer organisation and provides a basis for development of C. difficile-specific therapeutics.	Feb 2022

Instruments / Technical Area

Publication Details

Published

Actions

I04-Macromolecular Crystallography
I23-Long wavelength MX
I24-Microfocus Macromolecular Crystallography
Krios III-Titan Krios III at Diamond

Structure and assembly of the S-layer in C. difficile