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Instruments / Technical Area	Publication Details	Published	Actions
Krios I-Titan Krios I at Diamond	eIF6 rebinding dynamically couples ribosome maturation and translation Pekka Jaako , Alexandre Faille , Shengjiang Tan , Chi C. Wong , Norberto Escudero-Urquijo , Pablo Castro-Hartmann , Penny Wright , Christine Hilcenko , David J. Adams , Alan J. Warren Nature Communications 13 DOI: 10.1038/s41467-022-29214-7 Diamond Proposal Number(s): [22238] Open Access Show Abstract ▼ Abstract: Protein synthesis is a cyclical process consisting of translation initiation, elongation, termination and ribosome recycling. The release factors SBDS and EFL1—both mutated in the leukemia predisposition disorder Shwachman-Diamond syndrome — license entry of nascent 60S ribosomal subunits into active translation by evicting the anti-association factor eIF6 from the 60S intersubunit face. We find that in mammalian cells, eIF6 holds all free cytoplasmic 60S subunits in a translationally inactive state and that SBDS and EFL1 are the minimal components required to recycle these 60S subunits back into additional rounds of translation by evicting eIF6. Increasing the dose of eIF6 in mice in vivo impairs terminal erythropoiesis by sequestering post-termination 60S subunits in the cytoplasm, disrupting subunit joining and attenuating global protein synthesis. These data reveal that ribosome maturation and recycling are dynamically coupled by a mechanism that is disrupted in an inherited leukemia predisposition disorder.	Mar 2022

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Krios I-Titan Krios I at Diamond

eIF6 rebinding dynamically couples ribosome maturation and translation

Pekka Jaako , Alexandre Faille , Shengjiang Tan , Chi C. Wong , Norberto Escudero-Urquijo , Pablo Castro-Hartmann , Penny Wright , Christine Hilcenko , David J. Adams , Alan J. Warren

Nature Communications 13

DOI: 10.1038/s41467-022-29214-7

Diamond Proposal Number(s): [22238]