Diamond Publications - Search Results

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
Krios III-Titan Krios III at Diamond	Age-dependent formation of TMEM106B amyloid filaments in human brains Manuel Schweighauser , Diana Arseni , Mehtap Bacioglu , Melissa Huang , Sofia Lovestam , Yang Shi , Yang Yang , Wenjuan Zhang , Abhay Kotecha , Holly J. Garringer , Ruben Vidal , Grace I. Hallin , Kathy L. Newell , Airi Tarutani , Shigeo Murayama , Masayuki Miyazaki , Yuko Saito , Mari Yoshida , Kazuko Hasegawa , Tammaryn Lashley , Tamas Revesz , Gabor G. Kovacs , John Van Swieten , Masaki Takao , Masato Hasegawa , Bernardino Ghetti , Maria Grazia Spillantini , Benjamin Ryskeldi-Falcon , Alexey G. Murzin , Michel Goedert , Sjors H. W. Scheres Nature DOI: 10.1038/s41586-022-04650-z Diamond Proposal Number(s): [17434, 23268] Open Access Show Abstract ▼ Abstract: Many age-dependent neurodegenerative diseases, like Alzheimer’s and Parkinson’s, are characterised by abundant inclusions of amyloid filaments. Filamentous inclusions of the proteins tau, amyloid-β (Aβ), α-synuclein and TDP-43 are the most common1,2. Here, we used electron cryo-microscopy (cryo-EM) structure determination to show that residues 120-254 of the lysosomal type II transmembrane protein 106B (TMEM106B) also form amyloid filaments in human brains. We determined the cryo-EM structures of TMEM106B filaments from a number of brain regions of 22 individuals with abundant amyloid deposits, including sporadic and inherited tauopathies, Aβ-amyloidoses, synucleinopathies and TDP-43 proteinopathies, as well as from the frontal cortex of 3 neurologically normal individuals with no or only few amyloid deposits. We observed three TMEM106B folds, with no clear relationships between folds and diseases. TMEM106B filaments correlated with the presence of a 29 kDa sarkosyl-insoluble fragment and globular cytoplasmic inclusions, as detected by an antibody specific for the C-terminal region of TMEM106B. The identification of TMEM106B filaments in the brains of older, but not younger, neurologically normal individuals indicates that they form in an age-dependent manner.	Mar 2022

Instruments / Technical Area

Publication Details

Published

Actions

Krios III-Titan Krios III at Diamond

Age-dependent formation of TMEM106B amyloid filaments in human brains