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Instruments / Technical Area	Publication Details	Published	Actions
I03-Macromolecular Crystallography I04-Macromolecular Crystallography I23-Long wavelength MX	Light activation of Orange Carotenoid Protein reveals bicycle-pedal single-bond isomerization Volha U. Chukhutsina , James M. Baxter , Alisia Fadini , Rhodri M. Morgan , Matthew A. Pope , Karim Maghlaoui , Christian M. Orr , Armin Wagner , Jasper J. Van Thor Nature Communications 13 DOI: 10.1038/s41467-022-34137-4 Diamond Proposal Number(s): [17221] Open Access Show Abstract ▼ Abstract: Orange Carotenoid protein (OCP) is the only known photoreceptor which uses carotenoid for its activation. It is found exclusively in cyanobacteria, where it functions to control light-harvesting of the photosynthetic machinery. However, the photochemical reactions and structural dynamics of this unique photosensing process are not yet resolved. We present time-resolved crystal structures at second-to-minute delays under bright illumination, capturing the early photoproduct and structures of the subsequent reaction intermediates. The first stable photoproduct shows concerted isomerization of C9’-C8’ and C7’-C6’ single bonds in the bicycle-pedal (s-BP) manner and structural changes in the N-terminal domain with minute timescale kinetics. These are followed by a thermally-driven recovery of the s-BP isomer to the dark state carotenoid configuration. Structural changes propagate to the C-terminal domain, resulting, at later time, in the H-bond rupture of the carotenoid keto group with protein residues. Solution FTIR and UV/Vis spectroscopy support the single bond isomerization of the carotenoid in the s-BP manner and subsequent thermal structural reactions as the basis of OCP photoreception.	Oct 2022

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I03-Macromolecular Crystallography
I04-Macromolecular Crystallography
I23-Long wavelength MX

Light activation of Orange Carotenoid Protein reveals bicycle-pedal single-bond isomerization

Volha U. Chukhutsina , James M. Baxter , Alisia Fadini , Rhodri M. Morgan , Matthew A. Pope , Karim Maghlaoui , Christian M. Orr , Armin Wagner , Jasper J. Van Thor

Nature Communications 13

DOI: 10.1038/s41467-022-34137-4

Diamond Proposal Number(s): [17221]