Diamond Publications - Search Results

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I22-Small angle scattering & Diffraction	High resolution membrane structures within hybrid lipid-polymer vesicles revealed by combining X-ray scattering and electron microscopy Rashmi Seneviratne , Georgina Coates , Zexi Xu , Caitlin E. Cornell , Rebecca F. Thompson , Amin Sadeghpour , Daniel P. Maskell , Lars J. C. Jeuken , Michael Rappolt , Paul A. Beales Small 1863 DOI: 10.1002/smll.202206267 Diamond Proposal Number(s): [19127, 18027] Open Access Show Abstract ▼ Abstract: Hybrid vesicles consisting of phospholipids and block-copolymers are increasingly finding applications in science and technology. Herein, small angle X-ray scattering (SAXS) and cryo-electron tomography (cryo-ET) are used to obtain detailed structural information about hybrid vesicles with different ratios of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and poly(1,2-butadiene-block-ethylene oxide) (PBd22-PEO14, Ms = 1800 g mol−1). Using single particle analysis (SPA) the authors are able to further interpret the information gained from SAXS and cryo-ET experiments, showing that increasing PBd22-PEO14 mole fraction increases the membrane thickness from 52 Å for a pure lipid system to 97 Å for pure PBd22-PEO14 vesicles. Two vesicle populations with different membrane thicknesses in hybrid vesicle samples are found. As these lipids and polymers are reported to homogeneously mix, bistability is inferred between weak and strong interdigitation regimes of PBd22-PEO14 within the hybrid membranes. It is hypothesized that membranes of intermediate structure are not energetically favorable. Therefore, each vesicle exists in one of these two membrane structures, which are assumed to have comparable free energies. The authors conclude that, by combining biophysical methods, accurate determination of the influence of composition on the structural properties of hybrid membranes is achieved, revealing that two distinct membranes structures can coexist in homogeneously mixed lipid-polymer hybrid vesicles.	Mar 2023

Instruments / Technical Area

Publication Details

Published

Actions

I22-Small angle scattering & Diffraction

High resolution membrane structures within hybrid lipid-polymer vesicles revealed by combining X-ray scattering and electron microscopy

Rashmi Seneviratne , Georgina Coates , Zexi Xu , Caitlin E. Cornell , Rebecca F. Thompson , Amin Sadeghpour , Daniel P. Maskell , Lars J. C. Jeuken , Michael Rappolt , Paul A. Beales

Small 1863

DOI: 10.1002/smll.202206267

Diamond Proposal Number(s): [19127, 18027]