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Instruments / Technical Area	Publication Details	Published	Actions
B23-Circular Dichroism I04-Macromolecular Crystallography	A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide Gregor Hagelueken , Bradley R Clarke , Hexian Huang , Anne Tuukkanen , Iulia Danciu , Dmitri I Svergun , Rohanah Hussain , Huanting Liu , Chris Whitfield , James Naismith Nature Structural & Molecular Biology DOI: 10.1038/nsmb.2935 PMID: 25504321 Diamond Proposal Number(s): [11051, 12036] Open Access Show Abstract ▼ Abstract: Long-chain bacterial polysaccharides have important roles in pathogenicity. In Escherichia coli O9a, a model for ABC transporter–dependent polysaccharide assembly, a large extracellular carbohydrate with a narrow size distribution is polymerized from monosaccharides by a complex of two proteins, WbdA (polymerase) and WbdD (terminating protein). Combining crystallography and small-angle X-ray scattering, we found that the C-terminal domain of WbdD contains an extended coiled-coil that physically separates WbdA from the catalytic domain of WbdD. The effects of insertions and deletions in the coiled-coil region were analyzed in vivo, revealing that polymer size is controlled by varying the length of the coiled-coil domain. Thus, the coiled-coil domain of WbdD functions as a molecular ruler that, along with WbdA:WbdD stoichiometry, controls the chain length of a model bacterial polysaccharide.polymerized from monosaccharides by a complex of two proteins, WbdA (polymerase) and WbdD (terminating protein). Combining crystallography and small-angle X-ray scattering, we found that the C-terminal domain of WbdD contains an extended coiled-coil that physically separates WbdA from the catalytic domain of WbdD. The effects of insertions and deletions in the coiled-coil region were analyzed in vivo, revealing that polymer size is controlled by varying the length of the coiled-coil domain. Thus, the coiled-coil domain of WbdD functions as a molecular ruler that, along with WbdA:WbdD stoichiometry, controls the chain length of a model bacterial polysaccharide.	Dec 2014

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B23-Circular Dichroism
I04-Macromolecular Crystallography

A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide

Gregor Hagelueken , Bradley R Clarke , Hexian Huang , Anne Tuukkanen , Iulia Danciu , Dmitri I Svergun , Rohanah Hussain , Huanting Liu , Chris Whitfield , James Naismith

Nature Structural & Molecular Biology

DOI: 10.1038/nsmb.2935

PMID: 25504321

Diamond Proposal Number(s): [11051, 12036]