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Instruments / Technical Area	Publication Details	Published	Actions
I02-Macromolecular Crystallography I03-Macromolecular Crystallography I04-Macromolecular Crystallography	Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus Carey Lambert , Ian T. Cadby , Rob Till , Nhat Khai Bui , Thomas R. Lerner , William S. Hughes , David J. Lee , Luke J. Alderwick , Waldemar Vollmer , Elizabeth R. Sockett , Andrew L Lovering Nature Communications 6 DOI: 10.1038/ncomms9884 PMID: 26626559 Diamond Proposal Number(s): [8359, 10369] Open Access Show Abstract ▼ Abstract: Predatory Bdellovibrio bacteriovorus are natural antimicrobial organisms, killing other bacteria by whole-cell invasion. Self-protection against prey-metabolizing enzymes is important for the evolution of predation. Initial prey entry involves the predator's peptidoglycan DD-endopeptidases, which decrosslink cell walls and prevent wasteful entry by a second predator. Here we identify and characterize a self-protection protein from B. bacteriovorus, Bd3460, which displays an ankyrin-based fold common to intracellular pathogens of eukaryotes. Co-crystal structures reveal Bd3460 complexation of dual targets, binding a conserved epitope of each of the Bd3459 and Bd0816 endopeptidases. Complexation inhibits endopeptidase activity and cell wall decrosslinking in vitro. Self-protection is vital - ΔBd3460 Bdellovibrio deleteriously decrosslink self-peptidoglycan upon invasion, adopt a round morphology, and lose predatory capacity and cellular integrity. Our analysis provides the first mechanistic examination of self-protection in Bdellovibrio, documents protection-multiplicity for products of two different genomic loci, and reveals an important evolutionary adaptation to an invasive predatory bacterial lifestyle	Dec 2015

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I02-Macromolecular Crystallography
I03-Macromolecular Crystallography
I04-Macromolecular Crystallography

Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus