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B23-Circular Dichroism	Chaperone-like effect of ceftriaxone on HEWL aggregation: A spectroscopic and computational study Paolo Ruzza , Rosa Maria Vitale , Rohanah Hussain , Alessia Montini , Claudia Honisch , Alice Pozzebon , Charlotte S. Hughes , Barbara Biondi , Pietro Amodeo , Gianpietro Sechi , Giuliano Siligardi Biochimica Et Biophysica Acta (bba) - General Subjects DOI: 10.1016/j.bbagen.2018.02.014 Diamond Proposal Number(s): [8034] Show Abstract ▼ Abstract: Background: Lysozyme is a widely distributed enzyme present in a variety of tissue and body fluids. Human and hen egg white lysozyme are used as validated model to study protein folding and stability and to understand protein misfolding and aggregation. We recently found that ceftriaxone, a β-lactam antibiotic able to overcome the blood-brain barrier, successfully eliminated the cellular toxic effects of misfolded proteins as Glial Fibrillary Acidic Protein and α-synuclein. To further understand the anti-amyloidogenic properties of ceftriaxone, we studied its activity towards lysozyme aggregation with the aim to investigate a possible chaperone-like activity of this molecule. Methods: Here we present the results obtained from fluorescence and synchrotron radiation circular dichroism spectroscopies and from molecular docking and molecular dynamics about the lysozyme-ceftriaxone interaction at neutral and acidic pH values. Results: We found that ceftriaxone exhibits comparable affinity constants to lysozyme in both experimental pH conditions and that its addition enhanced lysozyme stability reducing its aggregation propensity in acidic conditions. Computational methods allowed the identification of the putative binding site of ceftriaxone, thus rationalizing the spectroscopic results. Conclusions: Spectroscopy data and molecular dynamics indicated a protective effect of ceftriaxone on pathological aggregation phenomena suggesting a chaperone-like effect of this molecule on protein folding. General significance: These results, in addition to our previous studies on α-synuclein and GFAP, confirm the property of ceftriaxone to inhibit the pathological protein aggregation of lysozyme also by a chaperone-like mechanism, extending the potential therapeutic application of this molecule to some forms of human hereditary systemic amyloidosis.	Mar 2018

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B23-Circular Dichroism

Chaperone-like effect of ceftriaxone on HEWL aggregation: A spectroscopic and computational study